Application of Elastography for the Noninvasive Assessment of Biomechanics in Engineered Biomaterials and Tissues.
Application of Elastography for the Noninvasive Assessment of Biomechanics in Engineered Biomaterials and Tissues.
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DOI：
10.1007/s10439-015-1542-x
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发表时间：
2016-03
影响因子：
3.8
通讯作者：
Neu CP
中科院分区：
文献类型：
--
作者：
Kim W;Ferguson VL;Borden M;Neu CP
工程生物材料和组织的弹性特性影响其植入后修复潜力和结构完整性，并且对于帮助调节细胞命运和基因表达至关重要。性能的测量（例如，刚度或剪切模量）可以使用弹性成像来获得，弹性成像利用非侵入性成像模态来提供指示再生状态的材料的功能信息。在这篇综述中，我们概述了目前领先的弹性成像方法，可用于表征生物材料和组织的性能，适用于修复和机械生物学研究。我们描述的方法，利用磁共振，超声，光学相干弹性成像，突出其潜在的纵向监测植入材料在体内，除了时空的限制，每种方法探测的变化，在细胞负载的结构。微弹性成像方法现在允许在二维和三维中以接近5-100 μm的长度尺度进行采集。因此，本文介绍的许多方法能够对接近细胞尺度的生物材料和组织进行纵向监测。然而，关键因素，如各向异性，异质性和粘弹性-固有的许多软组织-往往没有得到充分的描述，因此需要进一步的进步和未来的发展。
The elastic properties of engineered biomaterials and tissues impact their post-implantation repair potential and structural integrity, and are critical to help regulate cell fate and gene expression. The measurement of properties (e.g., stiffness or shear modulus) can be attained using elastography, which exploits noninvasive imaging modalities to provide functional information of a material indicative of the regeneration state. In this review, we outline the current leading elastography methodologies available to characterize the properties of biomaterials and tissues suitable for repair and mechanobiology research. We describe methods utilizing magnetic resonance, ultrasound, and optical coherent elastography, highlighting their potential for longitudinal monitoring of implanted materials in vivo, in addition to spatiotemporal limits of each method for probing changes in cell-laden constructs. Micro-elastography methods now allow acquisitions at length scales approaching 5–100 μm in two and three dimensions. Many of the methods introduced in this review are therefore capable of longitudinal monitoring in biomaterials and tissues approaching the cellular scale. However, critical factors such as anisotropy, heterogeneity and viscoelasity—inherent in many soft tissues—are often not fully described and therefore require further advancements and future developments.