Hepatic amino acid-dependent signaling is under the control of AMP-dependent protein kinase
Hepatic amino acid-dependent signaling is under the control of AMP-dependent protein kinase
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DOI：
10.1016/s0014-5793(02)02815-6
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发表时间：
2002-06-19
期刊：
影响因子：
3.5
通讯作者：
Meijer, AJ
中科院分区：
文献类型：
--
作者：
Dubbelhuis, PF;Meijer, AJ
近年来越来越清楚的是，氨基酸可以刺激信号转导途径，导致雷帕霉素下游靶点的哺乳动物靶点磷酸化。我们现在发现，当 AMP 依赖性蛋白激酶 (AMPK) 被嘌呤核糖核苷类似物 AICAriboside、果糖或甘油激活时，肝细胞中 p70S6 激酶和 S6 的氨基酸依赖性磷酸化被阻止。蛋白激酶 B 的胰岛素依赖性磷酸化不受 AMPK 激活的影响。当 AMPK 被激活时，蛋白质合成会受到强烈抑制。结论是，氨基酸依赖性信号，一种蛋白质合成代谢信号，可以通过 AMPK 的激活来有效拮抗。 (C) 2002 年由 Elsevier Science B.V. 代表欧洲生化学会联合会出版。
It has become increasingly clear in recent years that amino acids can stimulate a signal transduction pathway resulting in the phosphorylation of mammalian target of rapamycin downstream targets. We have now found that amino acid-dependent phosphorylation of p70S6 kinase and of S6 in hepatocytes is prevented when AMP-dependent protein kinase (AMPK) is activated by either the purine ribonucleoside analogue AICAriboside, fructose or glycerol. Insulin-dependent phosphorylation of protein kinase B is not affected by AMPK activation. Protein synthesis is strongly inhibited when AMPK is activated. It is concluded that amino acid-dependent signaling, a protein-anabolic signal, can be effectively antagonized by activation of AMPK. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.