RBC membrane camouflaged boron nitride nanospheres for enhanced biocompatible performance
RBC membrane camouflaged boron nitride nanospheres for enhanced biocompatible performance
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红细胞膜伪装氮化硼纳米球可增强生物相容性
DOI：
10.1016/j.colsurfb.2020.110964
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发表时间：
2020-06-01
影响因子：
5.8
通讯作者：
Zhang, Huijie
中科院分区：
文献类型：
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作者：
Feng, Shini;Li, Hui;Zhang, Huijie
氮化硼纳米球由于其独特的物理化学性质在许多领域引起了广泛的关注。BNNS的生物医学应用最近也进行了探索。然而，由于BNNS的疏水性和分散性差，其生物相容性特别是体内生物安全性的研究报道很少，目前尚不清楚。本工作首次采用物理挤压法将BNNS与红细胞膜包埋在一起。然后将CM-BNNS与细胞一起孵育，并静脉注射到小鼠体内，以揭示其体外和体内毒性的潜力。结果是有希望的，因为与原始BNNS相比，CM-BNNS表现出更好的分散性和稳定性。体外实验表明，CM-BNNS的生物安全性相对增强。红细胞膜涂层赋予BNNS显着延长血液循环和减少在肺中的积累。此外，CM-BNNS在10 mg/kg剂量下对所有评价的血液参数和治疗小鼠的组织均无不良影响。总之，我们的工作证明了CM-BNNS的最佳生物相容性，并为其未来的生物医学应用铺平了道路。
Boron nitride nanospheres (BNNS) have attracted increasing attention in many fields due to their unique physicochemical properties. Biomedical application of BNNS has also been explored recently. However, limited by the hydrophobicity and poor dispersity of BNNS, their biocompatible performance especially the in vivo biosafety has rarely been reported and is still unclear now. In this work, BNNS were firstly camouflaged with red blood cell membrane by physical extrusion (CM-BNNS). CM-BNNS were then incubated with cells as well as intravenously injected into the mice to uncover their potential in vitro and in vivo toxicity. Results were promising as CM-BNNS exhibited better dispersion and stability compared with pristine BNNS. In vitro data demonstrated the relatively enhanced biosafety of CM-BNNS. The red blood cell membrane coating endowed BNNS with markedly prolonged blood circulation and decreased accumulation in the lung. In addition, CM-BNNS showed no adverse effects on all the evaluated hematic parameters and tissues of treated mice at a dose of 10 mg/kg. Taken together, our work demonstrated the optimal biocompatibility of CM-BNNS and pave the way for their future biomedical applications.