The Sm Complex Is Required for the Processing of Non-Coding RNAs by the Exosome
The Sm Complex Is Required for the Processing of Non-Coding RNAs by the Exosome
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DOI：
10.1371/journal.pone.0065606
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发表时间：
2013-06-06
期刊：
影响因子：
3.7
通讯作者：
Vasiljeva, Lidia
中科院分区：
文献类型：
--
作者：
Coy, Sarah;Volanakis, Adam;Vasiljeva, Lidia
RNA调控领域的一个关键问题是一些外泌体底物，如剪接体snRNA和端粒酶RNA，如何逃避降解并加工成稳定的功能性RNA分子。这些非编码RNA的典型特征是在成熟RNA分子的3 '端存在Sm复合物。在这里，我们报告说，在酿酒酵母中存在完整的Sm结合位点是必需的外泌体介导的加工端粒酶RNA从多聚腺苷酸化的前体到其成熟的形式，是必不可少的延长端粒的功能。此外，我们证明了相同的途径参与snRNA的成熟。此外，将Sm结合位点插入通常被外泌体完全破坏的不稳定RNA中，导致其部分稳定化。我们还表明，端粒酶RNA积累裂殖酵母粟酒裂殖酵母外泌体突变体，表明保守的作用外泌体在加工和降解端粒酶RNA。总之，我们的数据提供了重要的外泌体依赖的RNA加工以及端粒酶RNA生物合成的调节机制的见解。
A key question in the field of RNA regulation is how some exosome substrates, such as spliceosomal snRNAs and telomerase RNA, evade degradation and are processed into stable, functional RNA molecules. Typical feature of these non-coding RNAs is presence of the Sm complex at the 3 ' end of the mature RNA molecule. Here, we report that in Saccharomyces cerevisiae presence of intact Sm binding site is required for the exosome-mediated processing of telomerase RNA from a polyadenylated precursor into its mature form and is essential for its function in elongating telomeres. Additionally, we demonstrate that the same pathway is involved in the maturation of snRNAs. Furthermore, the insertion of an Sm binding site into an unstable RNA that is normally completely destroyed by the exosome, leads to its partial stabilization. We also show that telomerase RNA accumulates in Schizosaccharomyces pombe exosome mutants, suggesting a conserved role for the exosome in processing and degradation of telomerase RNA. In summary, our data provide important mechanistic insight into the regulation of exosome dependent RNA processing as well as telomerase RNA biogenesis.