Automated method for tracking individual red blood cells within capillaries to compute velocity and oxygen saturation
Automated method for tracking individual red blood cells within capillaries to compute velocity and oxygen saturation
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DOI：
10.1080/10739680591003341
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发表时间：
2005-09-01
期刊：
影响因子：
2.4
通讯作者：
Ellis, CG
中科院分区：
文献类型：
--
作者：
Japee, SA;Pittman, RN;Ellis, CG
目的：作者提出了一种新的方法来跟踪单个红细胞（RBC），因为他们通过毛细血管移动。该方法使用最近开发的测量和分析系统的毛细血管氧运输（MASCOT）和时空图像的概念，以跟踪红细胞之间的连续帧的视频recordings of the microcirculation.Methods：在时空图像显示在一个单一的静态图像为一个单一的毛细管的所有红细胞的位置作为时间的函数。分析BBC的录像带如何通过毛细血管，以获得单个细胞穿过感兴趣的毛细血管时的速度。产生时空图像以跟踪红细胞从一个家庭到下一个家庭，并计算它们的速度。基于从同步录像带在两个波长下获得的每个细胞的光密度值，可以确定细胞的氧饱和度。以这种方式，氧饱和度可以跟踪相同的细胞，因为他们通过capillary.Results和结论：这些测量，采取在一起，让一个确定有多少和多快的氧气被输送到周围组织。该方法首次提供了一种跟踪流经毛细血管网络的单个RBC并研究其RBC动力学和氧合的方法。
Objective: The authors present a new method to track individual red blood cells (RBCs) as they move through capillaries. This method uses a recently developed Measurement and Analysis System for Capillary Oxygen Transport (MASCOT) and the concept of space-time images to track RBCs between consecutive frames of video recordings of the microcirculation.Methods: At space-time image displays in a single static image for a single capillary the location of all RBCs as a function of time. Analysis is performed on video tapes of BBC How through capillaries to obtain velocity of individual cells as they traverse be capillary of interest A space-time image is generated to track RBCs from one home to the next and their velocities are computed Based on the optical density values of each cell obtained from synchronized videotapes at two wavelengths, the oxygen saturation of a cell can be determined. In this manner, oxygen saturation can be tracked for the same cells as they move through the capillary.Results and Conclusions: These measurements, taken together, allow one to determine how much and how fast oxygen is being delivered to the surrounding tissue. This method provides, for the first time, a way to track individual RBCs flowing through capillary networks and study their RBC dynamics and oxygenation.