Accuracy of calculated free testosterone differs between equations and depends on gender and SHBG concentration
Accuracy of calculated free testosterone differs between equations and depends on gender and SHBG concentration
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DOI：
10.1016/j.steroids.2010.08.008
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发表时间：
2011-01-01
期刊：
影响因子：
2.7
通讯作者：
Singh, Ravinder J.
中科院分区：
文献类型：
--
作者：
Hackbarth, Jennifer S.;Hoyne, Jonathan B.;Singh, Ravinder J.
通常计算血清游离睾酮（fT）浓度，但不同的方程往往产生不一致的结果。本研究探讨了这种差异性的来源。我们比较了三个建立和两个新的方程，不同的只是他们的睾酮结合常数与同位素稀释平衡透析在两个不同性别分布的患者组。检查方程组件，以确定它们如何影响与同位素稀释平衡透析的相关性。每个患者组的相关常数与该组的同位素稀释平衡透析相关性最好，而不是另一组。同位素稀释平衡透析和计算的fT结果之间相关性最差的样本具有显著较高的SHBG浓度。无论方程如何，>= 25%的样品显示出与同位素稀释平衡透析的不可接受的偏差。用于建立方程的患者组的关联常数和性别构成以及SHBG浓度均显著影响与同位素稀释平衡透析的相关性。因此，将许多fT方程应用于更广泛的人群，经常会产生与同位素稀释平衡透析有很大不同的结果。(C)2010年爱思唯尔公司All rights reserved.
Serum free testosterone (fT) concentrations are often calculated, however different equations often yield discrepant results. This study explores the sources of this variability. We compared three established and two new equations that differed only by their testosterone association constants with isotope dilution equilibrium dialysis in two patient groups with different gender distributions. Equation components were examined to determine how they impacted correlation with isotope dilution equilibrium dialysis. Association constants derived for each patient group correlated best with isotope dilution equilibrium dialysis for that group and not the other set. Samples with the poorest correlation between isotope dilution equilibrium dialysis and calculated fT results had significantly higher SHBG concentrations. Regardless of equation, >= 25% of samples showed unacceptable deviation from isotope dilution equilibrium dialysis. Association constants and gender makeup and SHBG concentration of the patient groups used to establish an equation all significantly impact correlation with isotope dilution equilibrium dialysis. Application of many fT equations to wider populations will therefore frequently yield results that differ substantially from isotope dilution equilibrium dialysis. (C) 2010 Elsevier Inc. All rights reserved.