The picornavirus avian encephalomyelitis virus possesses a hepatitis C virus-like internal ribosome entry site element
The picornavirus avian encephalomyelitis virus possesses a hepatitis C virus-like internal ribosome entry site element
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DOI：
10.1128/jvi.01957-07
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发表时间：
2008-02-01
影响因子：
5.4
通讯作者：
Roberts, Lisa O.
中科院分区：
文献类型：
--
作者：
Bakhshesh, Mehran;Groppelli, Elisabetta;Roberts, Lisa O.
禽脑脊髓炎病毒（AEV）是一种小核糖核酸病毒，在世界范围内引起家禽疾病，必须接种疫苗才能保护家禽。AEV目前被归类为肝病毒属，因为它的蛋白质与甲型肝炎病毒（HAV）的蛋白质最密切相关。我们现在提供的证据表明，AEV基因组的494个核苷酸长的5'非翻译区包含一个内部核糖体进入位点（IRES）元件，该元件在体外和哺乳动物细胞中有效地发挥作用。与HAV IRES不同，AEV IRES相对较短，在存在裂解的eIF 4G的情况下发挥作用，并且对eIF 4A抑制剂具有抗性。这些特性让人想起最近在某些其他小核糖核酸病毒中发现的IRES元件，如猪捷申病毒1（PTV-1）。与PTV-1 IRES一样，AEV IRES在序列、功能和预测二级结构方面与丙型肝炎病毒（HCV）IRES具有显著相似性。此外，在AEV IRES的3'末端预测的假结结构的突变分析支持我们提出的二级结构。因此，AEV是在其基因组中含有HCV样IRES元件的小核糖核酸病毒的另一个例子，因此，根据这些发现，可能需要重新评估其在肝病毒属中的分类。
Avian encephalomyelitis virus (AEV) is a picornavirus that causes disease in poultry worldwide, and flocks must be vaccinated for protection. AEV is currently classified within the hepatovirus genus, since its proteins are most closely related to those of hepatitis A virus (HAV). We now provide evidence that the 494-nucleotide-long 5' untranslated region of the AEV genome contains an internal ribosome entry site (IRES) element that functions efficiently in vitro and in mammalian cells. Unlike the HAV IRES, the AEV IRES is relatively short and functions in the presence of cleaved eIF4G and it is also resistant to an inhibitor of eIF4A. These properties are reminiscent of the recently discovered class of IRES elements within certain other picornaviruses, such as porcine teschovirus 1 (PTV-1). Like the PTV-1 IRES, the AEV IRES shows significant similarity to the hepatitis C virus (HCV) IRES in sequence, function, and predicted secondary structure. Furthermore, mutational analysis of the predicted pseudoknot structure at the 3' end of the AEV IRES lends support to the secondary structure we present. AEV is therefore another example of a picornavirus harboring an HCV-Iike IRES element within its genome, and thus, its classification within the hepatovirus genus may need to be reassessed in light of these findings.