Cell separation mediated by differential rolling adhesion
Cell separation mediated by differential rolling adhesion
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DOI：
10.1002/bit.1043
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发表时间：
2001-04-20
影响因子：
3.8
通讯作者：
Hammer, DA
中科院分区：
文献类型：
--
作者：
Greenberg, AW;Hammer, DA
最近，我们发现了造血干细胞和祖细胞在发育过程中的成熟度与选择素的滚动效率之间的相关性。这些发现促使我们探索一种新的分离方法，该方法利用了细胞群体之间选择素介导的滚动粘附的差异。我们扩展了之前开发的无细胞系统的使用，以研究唾液酸刘易斯x（sLe（x））涂层微球群体的分离，这些微球设计成在明确定义的流动下以不同的平均速度在L-选择素嵌合基质上滚动。结果表明，利用细胞或微球群体之间的平均滚动速度的差异的分离是可实现的。对于较慢的轧制，或更理想的，群体获得了优异的回收率和纯度值，并且可以从轧制速度测量中估计。我们还评估了选择素介导的成人骨髓细胞群的分离的可行性，使用先前获得的滚动速度和滚动流量数据的CD 34（+）和CD 34（-）成人骨髓细胞的L-选择素基板。我们相信，通过微分滚动粘附介导的细胞分离可用于从成人骨髓细胞制备物中富集造血干细胞和祖细胞群体，并且该方法具有优于现有抗体介导的细胞亲和层析技术的几个主要优点。(C)John Wiley & Sons，Inc.
Recently, we showed a correlation between the maturity of hematopoietic stem and progenitor cells during development and rolling efficiency on selectins. These findings motivated us to explore a novel separation that exploits differences in selectin-mediated rolling adhesion between populations of cells. We extend the use of a previously developed cell-free system to study the separation of populations of sialyl Lewis x (sLe(x))-coated microspheres designed to roll with different average velocities on L-selectin chimeric substrates under well-defined flow. Results show that a separation that exploits differences in average rolling velocities between cell or microsphere populations is attainable. Excellent recovery and purity values for the slower rolling, or more desirable, populations are obtained and can be estimated from rolling velocity measurements. We also assess the feasibility of a selectin-mediated separation of adult bone marrow cell populations using previously obtained rolling velocity and rolling flux data for CD34(+) and CD34(-) adult bone marrow cells on L-selectin substrates. We believe that a cell separation mediated by differential rolling adhesion can be used to enrich populations of hematopoietic stem and progenitor cells from an adult bone marrow cell preparation and that this method possesses several major advantages over existing antibody-mediated cell-affinity chromatography technologies. (C) 2001 John Wiley & Sons, Inc.