Nicotinic antagonist α-bungarotoxin binding to rat hippocampal neurons containing nitric oxide synthase
Nicotinic antagonist α-bungarotoxin binding to rat hippocampal neurons containing nitric oxide synthase
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DOI：
10.1016/s0006-8993(97)00978-5
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发表时间：
1997-11-21
期刊：
影响因子：
2.9
通讯作者：
Freedman, R
中科院分区：
文献类型：
--
作者：
Adams, CE;Freedman, R
海马是α-银环蛇毒素（α-BTX）结合的主要靶点。该配体与α 7烟碱胆碱能受体结合，该受体与海马对重复听觉刺激的习惯化有关，这种现象被认为涉及抑制性神经元。本研究检测了α-BTX是否与含有一氧化氮合酶（NOS）的神经元结合，NOS是抑制性海马神经元的一个亚群的标志物。对大鼠海马切片进行NOS免疫组织化学处理，拍照，然后进行[I-125]alpha-BTX放射自显影。神经元的分布之间的比较NOS和那些阳性的α-BTX结合在海马结构的相同区域的免疫反应，揭示了不同程度的共定位的NOS和α-BTX。在用α-BTX标记的细胞中，齿状回中的2%和海马中的40%也对NOS呈免疫反应性。这些NOS/alpha-BTX神经元在CA 1层中最普遍。这些结果提示，NOS神经元可能在α 7介导的大鼠海马对重复听觉刺激的抑制中发挥作用。(C)1997年Elsevier Science B.V.
The hippocampus is a major target of alpha-bungarotoxin (alpha-BTX) binding. This ligand binds to the alpha 7 nicotinic, cholinergic receptor, which has been implicated in hippocampal habituation to repetitive auditory stimulation, a phenomenon thought to involve inhibitory neurons. This study examined whether alpha-BTX binds to neurons containing nitric oxide synthase (NOS), a marker of one subgroup of inhibitory hippocampal neurons. Rat hippocampal sections were processed for NOS immunohistochemistry, photographed and then processed for [I-125]alpha-BTX autoradiography. Comparison between the distribution of neurons immunoreactive for NOS and those positive for alpha-BTX binding in the same regions of the hippocampal formation revealed a variable degree of colocalization of NOS and alpha-BTX. Of the cells labeled with alpha-BTX, 2% in the dentate gyrus and 40% in the hippocampus proper were also immunoreactive for NOS. These NOS/alpha-BTX neurons were most prevalent in CA1 stratum oriens. The results suggest a possible role for NOS-containing neurons in alpha 7-mediated inhibition to repetitive auditory stimulation in rat hippocampus. (C) 1997 Elsevier Science B.V.