Quantitative real-time catheter-based fluorescence molecular imaging in mice
Quantitative real-time catheter-based fluorescence molecular imaging in mice
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DOI：
10.1148/radiol.2452061613
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发表时间：
2007-11-01
期刊：
影响因子：
19.7
通讯作者：
Mahmood, Umar
中科院分区：
文献类型：
--
作者：
Upadhyay, Rabi;Sheth, Rahul A.;Mahmood, Umar
目的：前瞻性地评价一种光学成像系统，该系统设计用于对荧光分子探针进行定量的、基于活体导管的成像。材料和方法：本研究是根据机构动物护理委员会批准的方案进行的。为实现实时定量近红外成像的归一化算法，研制了一套光纤导管成像系统。该系统通过对荧光色素模体的体外成像和在注射酶激活的近红外探针后在小鼠腹部移植的肿瘤(n=7)中获得的体内荧光测量来验证。使用标准的方差分析检验来确定来自不同荧光浓度的信号的显著差异。结果：放置在不同近红外荧光浓度孔上方的光纤导管测量的原始近红外荧光强度随导管位置的不同而显著(100%)变化，而校正后的近红外信号被限制在其平均值的10%范围内，且具有显著差异(P
Purpose:To prospectively evaluate an optical imaging system designed to perform quantitative, intravital catheter- based imaging of fluorescent molecular probes.Materials and Methods: This study was performed according to a protocol approved by the institutional animal care committee. A fiberoptic catheter imaging system was developed to implement a normalization algorithm for real- time quantitative nearinfrared ( NIR) imaging. The system was validated with in vitro imaging of fluorochrome phantoms and in vivo fluorescence measurements obtained in tumors implanted in murine abdomens ( n = 7) after administration of an enzyme- activatable NIR probe. Standard analysis of variance tests were used to determine significant dissimilarities in signal from distinct fluorochrome concentrations. The clinical utility of the system was further evaluated by imaging orthotopically implanted murine colonic adenocarcinomas ( n = 4).Results: Raw NIR fluorescence intensities, which were measured with a fiberoptic catheter placed above wells of varying NIR fluorochrome concentration, varied markedly ( > 100%) with catheter position, while the corrected NIR signal was confined to a range of values within 10% of their mean for each individual fluorochrome concentration and were significantly distinct ( P