Multivitamins preventive therapy against subclinical endometritis in buffaloes: Its correlation to NEFA and oxidative stress
Multivitamins preventive therapy against subclinical endometritis in buffaloes: Its correlation to NEFA and oxidative stress
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DOI：
10.1016/j.repbio.2017.05.008
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发表时间：
2017-09-01
影响因子：
2.1
通讯作者：
El-Beltagy, Marwa A.
中科院分区：
文献类型：
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作者：
Ahmed, Eman A.;Elsayed, Doaa H.;El-Beltagy, Marwa A.
本研究旨在阐明维生素 AD(3)E 对水牛亚临床子宫内膜炎 (SCE) 发病率的体内抗氧化、预防和改善作用。二十四头水牛被平均分成两组； I组：对照组，II组：接受AD3E组合。使用细胞刷收集子宫内膜细胞学样本 (n = 48)，通过计算产后第 5 周 (W5) 时≥ 6% 的多形核细胞 (PMN) 和产后第 7 周 (W7) 时≥ 4% 的多形核细胞 (PMN) 来诊断 SCE。结果显示，AD3E组第7周血清超氧化物歧化酶和一氧化氮分别显着升高和降低。 PMN% 与第 5 周和第 7 周的氧化/抗氧化应激标记物显着相关。对照组水牛的阴道评分、PMN% 和血液中性粒细胞显着高于富含 AD3E 的水牛。因此，与对照水牛相比，补充 AD3E 的水牛在第 5 周（23.15% 和 38.46%）和第 7 周（9.8% 和 32.34%）的 SCE 患病率显着降低。对照组显示出较高的 NEFA 水平（P
The current study was designed to elucidate the in vivo antioxidant, preventive, and ameliorating effects of vitamins AD(3)E on the incidence of subclinical endometritis (SCE) in buffaloes. Twenty-four buffaloes were divided equally into two groups; group I: control and group II: received AD3E combination. Endometrial cytological samples (n = 48) were collected using cytobrush to diagnose SCE by counting polymorphonuclear cells (PMN) >= 6% at 5th (W5) and >= 4% at 7th (W7) weeks postpartum. Results revealed that serum superoxide dismutase and nitric oxide significantly increased and decreased, respectively at W7 in AD3E group. The PMN% were significantly correlated with oxidative/anti-oxidative stress markers at W5 and W7. Vaginal score, PMN%, and blood neutrophils were significantly higher in the control group buffaloes than the AD3E enriched ones. Therefore, the prevalence of SCE reduced significantly in the AD3E supplemented buffaloes as compared to the control ones at W5 (23.15% and 38.46%) and W7 (9.8% and 32.34%), respectively. The control group revealed higher NEFA levels (P