Detection and partial characterisation of two antibacterial factors from the excretions/secretions of the medicinal maggot Lucilia sericata and their activity against methicillin-resistant Staphylococcus aureus (MRSA)
Detection and partial characterisation of two antibacterial factors from the excretions/secretions of the medicinal maggot Lucilia sericata and their activity against methicillin-resistant Staphylococcus aureus (MRSA)
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DOI：
10.1016/j.micinf.2004.08.011
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发表时间：
2004-11-01
影响因子：
5.8
通讯作者：
Ratcliffe, NA
中科院分区：
文献类型：
--
作者：
Bexfield, A;Nigam, Y;Ratcliffe, NA
蛆疗法是清洁感染和坏死伤口的一种简单而非常成功的方法。在治疗不愈合的伤口，特别是那些感染了多重耐药病原体耐甲氧西林金黄色葡萄球菌（MRSA）的伤口方面，使用蛆虫变得越来越重要。MRSA感染的治疗面临越来越大的挑战，最近发现了耐万古霉素的MRSA菌株，这促使人们寻找新的抗菌化合物，特别是对蛆虫治疗背后的有效抗菌机制的研究。在这项研究中，我们报道了蚕绿蝇（Lucilia sericata）的排泄物/分泌物（ES）在体外对MRSA表现出有效的、热稳定的、蛋白酶抗性的抗菌活性。我们描述了两种抗菌因子的初步特征，从天然丝毛杆菌ES。一个小,
Maggot therapy is a simple and highly successful method for cleansing infected and necrotic wounds. The use of maggots has become increasingly important in the treatment of non-healing wounds, particularly those infected with the multidrug-resistant pathogen, methicillin-resistant Staphylococcus aureus (MRSA). The increasing challenge concerning the treatment of MRSA infections and the recent finding of vancomycin-resistant strains of MRSA have elicited the search for novel antibacterial compounds and, in particular, investigations into the potent antibacterial mechanism(s) behind maggot therapy. In this study, we report that excretions/secretions (ES) from the blowfly, Lucilia sericata, exhibit potent, thermally stable, protease resistant antibacterial activity against MRSA in vitro. We describe the initial characterisation of two antibacterial factors from native ES of L. sericata. A small,