A graphene field-effect transistor as a molecule-specific probe of DNA nucleobases
A graphene field-effect transistor as a molecule-specific probe of DNA nucleobases
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DOI：
10.1038/ncomms7563
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发表时间：
2015-03-01
影响因子：
16.6
通讯作者：
Cervenka, Jiri
中科院分区：
文献类型：
--
作者：
Dontschuk, Nikolai;Stacey, Alastair;Cervenka, Jiri
快速可靠的DNA测序是生物医学研究的长期目标。基于石墨烯的电子传感器的最新进展表明，它们对吸附分子具有前所未有的敏感性，这为无标记DNA测序技术带来了巨大的希望。到目前为止，所提出的测序方法依赖于石墨烯电子设备探测分子与石墨烯表面特定相互作用的能力。在这里，我们实验地演示了使用石墨烯场效应管(GFET)作为探针来检测吸附在石墨烯表面上的单个DNA碱基的存在。我们发现，GFET能够在四个不同的DNA碱基吸附时测量不同的覆盖率依赖的电导信号；这一结果可以归因于界面偶极场的形成。通过比较实验的GFET结果和基于同步加速器的材料分析，可以预测最终的器件灵敏度，并评估用石墨烯进行单核酸基传感的可行性。
Fast and reliable DNA sequencing is a long-standing target in biomedical research. Recent advances in graphene-based electrical sensors have demonstrated their unprecedented sensitivity to adsorbed molecules, which holds great promise for label-free DNA sequencing technology. To date, the proposed sequencing approaches rely on the ability of graphene electric devices to probe molecular-specific interactions with a graphene surface. Here we experimentally demonstrate the use of graphene field-effect transistors (GFETs) as probes of the presence of a layer of individual DNA nucleobases adsorbed on the graphene surface. We show that GFETs are able to measure distinct coverage-dependent conductance signatures upon adsorption of the four different DNA nucleobases; a result that can be attributed to the formation of an interface dipole field. Comparison between experimental GFET results and synchrotron-based material analysis allowed prediction of the ultimate device sensitivity, and assessment of the feasibility of single nucleobase sensing with graphene.