Sequence and expression pattern of pax-6 are highly conserved between zebrafish and mice.
Sequence and expression pattern of pax-6 are highly conserved between zebrafish and mice.
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发表时间：
1992-03
期刊：
影响因子：
4.6
通讯作者：
Andreas W. Puschel;Peter Gruss;M. Westerfield
中科院分区：
文献类型：
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作者：
Andreas W. Puschel;Peter Gruss;M. Westerfield
尽管脊椎动物的早期胚胎发育模式存在明显差异，但它们具有许多共同的发育机制和控制基因，这表明它们在发育的某些阶段使用类似的遗传程序。为了验证这一观点，我们从斑马鱼Brachydanio rerio中分离并表征了一个这样的基因pax-6， pax基因家族的成员，并通过比较其在小鼠中的同源基因来确定该基因的结构和表达的进化保守性。我们发现了斑马鱼pax-6信息的两种选择性拼接形式。斑马鱼pax-6基因的序列和表达模式与其小鼠同源基因非常相似。Pax-6的表达始于神经发育早期。神经外胚层的一条细胞，包括前侧间脑和部分端脑，表达pax-6以及从第一斜球水平延伸到中枢神经系统后端的后脑和脊髓腹侧。在后来的发育中，大脑中更有限的区域包括眼睛、嗅球和脑垂体表达pax-6。中脑-后脑连接处的细胞表达eng基因，并且与邻近的pax-6区域被几个不表达任何基因的细胞分开，表明该区域存在复杂的细分。Pax-6的表达出现在细胞间信号传导被认为是重要的过程中，例如在眼睛的诱导和脊髓和大脑的区域化过程中，这表明它可能是介导诱导相互作用反应的一个组成部分。
Despite obvious differences in the patterns of early embryonic development, vertebrates share a number of developmental mechanisms and control genes, suggesting that they use similar genetic programs at some stages of development. To examine this idea, we isolated and characterized one such gene, pax-6, a member of the pax gene family, from the zebrafish Brachydanio rerio and determined the evolutionary conservation in the structure and expression of this gene by comparison to its homolog in mice. We found two alternatively spliced forms of the zebrafish pax-6 message. Sequence and expression pattern of the zebrafish pax-6 gene are remarkably similar to its murine homolog. pax-6 expression begins during early neurulation. A stripe of cells in the neuroectoderm, including the prospective diencephalon and a part of the telencephalon, expresses pax-6 as well as the hindbrain and the ventral spinal cord extending from the level of the first rhombomere to the posterior end of the CNS. During later development more limited regions of the brain including the eye, the olfactory bulb and the pituitary gland express pax-6. Cells at the midbrain-hindbrain junction express eng genes and are separated from the neighboring pax-6 regions by several cells that express neither gene, indicating a complex subdivision of this region. pax-6 expression appears during processes when cell-to-cell signalling is thought to be important, for example during induction of the eye and regionalization of the spinal cord and brain, suggesting that it may be one component mediating the response to inductive interactions.