In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration
In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration
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DOI：
10.1002/bit.24733
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发表时间：
2013-03-01
影响因子：
3.8
通讯作者：
Cost, Gregory J.
中科院分区：
文献类型：
--
作者：
Cristea, Sandra;Freyvert, Yevgeniy;Cost, Gregory J.
靶向DNA整合通常用于消除转基因表达的位置效应。整合可通过基于同源性和不依赖同源性的过程靶向基因组中的特定位点。这两种途径都是通过染色体上的位点特异性断裂来启动整合过程，通常由锌指核酸酶（ZFN）引起。我们之前描述了一种高效的不依赖同源性的靶向整合技术，该技术可捕获短的（……此处原文不完整）
Targeted DNA integration is commonly used to eliminate position effects on transgene expression. Integration can be targeted to specific sites in the genome via both homology-based and homology-independent processes. Both pathways start the integration process with a site-specific break in the chromosome, typically from a zinc-finger nuclease (ZFN). We previously described an efficient homology-independent targeted integration technique that captures short (