The role of buttonhead and Sp1 in the development of the ventral imaginal discs of Drosophila
The role of buttonhead and Sp1 in the development of the ventral imaginal discs of Drosophila
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DOI：
10.1242/dev.00832
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发表时间：
2003-12-01
期刊：
影响因子：
4.6
通讯作者：
Morata, G
中科院分区：
文献类型：
--
作者：
Estella, C;Rieckhof, G;Morata, G
相关基因buttonhead（btd）和果蝇SP1（果蝇同源的人类SP1基因）编码锌指转录因子，已知在果蝇头节和机械感觉幼虫器官的形成中发挥发育作用。我们报告了一个新的功能btd和Sp1：它们诱导的形成和腹侧成虫盘的生长所需的。它们作为headcase（hdc）和Distal-less（Dll）基因的激活剂，分配椎间盘原基的细胞。BTD和Sp1的需求在腹盘的发育过程中持续存在：RNA干扰导致腿和触角的大小大幅减少。btd在背侧成虫盘（眼、翅和背索）的异位表达导致相应的腹侧结构（触角和腿）的形成。然而，这些结构不是由存在于椎间盘中的形态发生信号形成的;表达btd的细胞产生它们自己的信号系统，包括建立一个清晰的表达边界，以及无翅基因和decapentaplegic基因的局部激活。因此，Btd产物具有诱导腹侧成虫盘发育所必需的整个遗传网络的活性的能力。我们建议，这种属性是btd/Sp1基因的初始功能的反映，包括建立腹盘原基的命运，并确定其模式和增长。
The related genes buttonhead (btd) and Drosophila Sp1 (the Drosophila homologue of the human SP1 gene) encode zinc-finger transcription factors known to play a developmental role in the formation of the Drosophila head segments and the mechanosensory larval organs. We report a novel function of btd and Sp1: they induce the formation and are required for the growth of the ventral imaginal discs. They act as activators of the headcase (hdc) and Distal-less (Dll) genes, which allocate the cells of the disc primordia. The requirement for btd and Sp1 persists during the development of ventral discs: inactivation by RNA interference results in a strong reduction of the size of legs and antennae. Ectopic expression of btd in the dorsal imaginal discs (eyes, wings and halteres) results in the formation of the corresponding ventral structures (antennae and legs). However, these structures are not patterned by the morphogenetic signals present in the dorsal discs; the cells expressing btd generate their own signalling system, including the establishment of a sharp boundary of engrailed expression, and the local activation of the wingless and decapentaplegic genes. Thus, the Btd product has the capacity to induce the activity of the entire genetic network necessary for ventral imaginal discs development. We propose that this property is a reflection of the initial function of the btd/Sp1 genes that consists of establishing the fate of the ventral disc primordia and determining their pattern and growth.