High efficiency transport of quantum dots into plant roots with the aid of silwet L-77
High efficiency transport of quantum dots into plant roots with the aid of silwet L-77
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借助 Silwet L-77 将量子点高效传输到植物根部
DOI：
10.1016/j.plaphy.2010.04.001
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发表时间：
2010-08-01
影响因子：
6.5
通讯作者：
Li, Lijia
中科院分区：
文献类型：
--
作者：
Hu, Yong;Li, Jun;Li, Lijia
量子点是一种新型的小分子、光稳定、明亮的荧光团，已经成功地应用于哺乳动物和人类的活细胞成像。本研究合成了高度分散的水溶性巯基乙酸(MAA)包裹的CdSe/ZnS量子点，它们适合作为荧光探针标记进行研究。用量子点处理玉米幼苗根的结果表明，表面活性剂L-77能有效地促进量子点向玉米根部的转运。在0.128~1.28mU M浓度范围内，量子点对玉米种子萌发和根生长的细胞毒性很低。在Hoagland溶液中加入氯化汞后，根组织中的qd含量下降，而加入β-硫醇则相反，这表明汞敏感过程在调节玉米根系qd流动中起着重要作用。我们推测，质外体途径对到达中柱的量子点的总量有很大贡献。因此，基于这种传输方法，MAA包覆的量子点可以用于植物系统中的实时成像，以验证已知的生理过程。(C)2010年爱思唯尔·马森公司。版权所有。
Quantum dots (QDs) are a novel type of small, photostable and bright fluorophores that have been successfully applied to mammalian and human live cell imaging. In this study, highly dispersive water-soluble mercaptoacetic acid (MAA)-coated CdSe/ZnS QDs were synthesized, which were suitable for investigation as fluorescent probe labels. The treatment of maize seedling roots with QDs showed that the surfactant silwet L-77 aided the efficient transport of QDs into maize roots. Under a concentration ranging from 0.128 to 1.28 mu M, QDs caused very low cytotoxicity on maize seed germination and root growth. The addition of mercuric chloride to the Hoagland solution resulted in a decrease of QD content in root tissues, and this decrease was reversed upon the addition of beta-mercaptoethanol, which suggests that mercury-sensitive processes play a significant role in regulating QD flow in the maize root system. We speculate that the apoplastic pathway can contribute substantially to the total quantity of QDs reaching the stele. Therefore, based on this transport approach, MAA-coated QDs can be utilized for live imaging in plant systems to verify known physiological processes. (C) 2010 Elsevier Masson SAS. All rights reserved.