Development of universal genetic markers based on single-copy orthologous (COSII) genes in Poaceae
Development of universal genetic markers based on single-copy orthologous (COSII) genes in Poaceae
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DOI：
10.1007/s00299-012-1371-4
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发表时间：
2013-03
影响因子：
6.2
通讯作者：
Hailan Liu;Xiaoqin Guo;Jiasheng Wu;Guo-Bo Chen;Yeqing Ying
中科院分区：
文献类型：
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作者：
Hailan Liu;Xiaoqin Guo;Jiasheng Wu;Guo-Bo Chen;Yeqing Ying
基于禾本科植物单拷贝同源（COSII）基因，建立了一套通用遗传标记。摘要COSII基因具有进化保守性，在物种间比较定位和系统发育研究中特别有用。本研究基于水稻、玉米、高粱、谷子和短柄草等5个禾科植物基因组序列，共鉴定出2684个COSII基因，并利用46对随机选择的引物，开发出1072个COSII标记，进一步评价了这些标记在5个竹种间的可转移性和多态性。在46个引物中，91.3%的引物在至少一个竹种中获得了明确的扩增，65.2%的引物在一个以上的竹种中产生了多态性。利用其中的42个品种构建了5种竹的系统发育关系，与基于营养形态的系统发育关系相比，系统发育关系可能更为精确。结果表明，这些标记在禾科植物遗传多样性和分类研究中具有广阔的应用前景。为了方便读者获取共同感兴趣的资讯，我们提供了一个基于网络的COSII标记数据库（http://www.sicau.edu.cn/web/yms/PCOSWeb/PCOS.html）。
Key messageWe develop a set of universal genetic markers based on single-copy orthologous (COSII) genes in Poaceae.AbstractBeing evolutionary conserved, single-copy orthologous (COSII) genes are particularly useful in comparative mapping and phylogenetic investigation among species. In this study, we identified 2,684 COSII genes based on five sequenced Poaceae genomes including rice, maize, sorghum, foxtail millet, and brachypodium, and then developed 1,072 COSII markers whose transferability and polymorphism among five bamboo species were further evaluated with 46 pairs of randomly selected primers. 91.3 % of the 46 primers obtained clear amplification in at least one bamboo species, and 65.2 % of them produced polymorphism in more than one species. We also used 42 of them to construct the phylogeny for the five bamboo species, and it might reflect more precise evolutionary relationship than the one based on the vegetative morphology. The results indicated a promising prospect of applying these markers to the investigation of genetic diversity and the classification of Poaceae. To ease and facilitate access of the information of common interest to readers, a web-based database of the COSII markers is provided ( http://www.sicau.edu.cn/web/yms/PCOSWeb/PCOS.html ).