Quantitative Trait Loci Associated with Foliar Trigonelline Accumulation in Glycine Max L.
Quantitative Trait Loci Associated with Foliar Trigonelline Accumulation in Glycine Max L.
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与大豆叶葫芦碱积累相关的数量性状位点。
DOI：
10.1155/s1110724302204039
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发表时间：
2002
影响因子：
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通讯作者：
Wood, Andrew J.
中科院分区：
文献类型：
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作者：
Cho, Youngkoo;Njiti, Victor N.;Chen, Xinbo;Triwatayakorn, Kanokporn;Kassem, My Abdelmajid;Meksem, Khalid;Lightfoot, David A.;Wood, Andrew J.
本研究的目的是利用大豆 RIL 群体 (1) 评估田间种植的大豆中叶葫芦碱 (TRG) 含量，(2) 确定 TRG 积累的遗传力，以及 (3) 识别与 TRG 积累中数量性状基因座 (QTL) 调节变异相关的 DNA 标记。 70 个重组自交系的频率分布显示，在豆荚发育阶段 (R4) 测量的 TRG 积累，在统计上没有显着偏离正态性 (P > .05)。六个不同的分子连锁基团 (LG)（B2、C2、D2、G、J 和 K）被鉴定为与叶 TRG 积累的 QTL 相关。两个不同连锁群上的两个独特的微卫星标记 (SSR) 鉴定出与叶 TRG 积累显着相关的 QTL：LG J (Satt285) 上的一个区域 (P = .0019，R2 = 15.9%) 和 LG C2 (Satt079) 上的第二个区域 (P = .0029，R2 = 13.4%)。
The objective of this study was to utilize a Glycine max RIL population to (1) evaluate foliar trigonelline (TRG) content in field-grown soybean, (2) determine the heritability of TRG accumulation, and (3) identify DNA markers linked to quantitative trait loci (QTLs) conditioning variation in TRG accumulation. Frequency distributions of 70 recombinant inbred lines showed statistically no significant departure from normality (P > .05) for TRG accumulation measured at pod development stage (R4). Six different molecular linkage groups (LGs) (B2, C2, D2, G, J, and K) were identified to be linked to QTLs for foliar TRG accumulation. Two unique microsatellite markers (SSR) on two different linkage groups identified QTL significantly associated with foliar TRG accumulation: a region on LG J (Satt285) (P = .0019, R2 = 15.9%) and a second region on LG C2 (Satt079) (P = .0029, R2 = 13.4%).