On-chip neural cell cultivation using agarose-microchamber array constructed by a photothermal etching method
On-chip neural cell cultivation using agarose-microchamber array constructed by a photothermal etching method
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DOI：
10.1002/eej.10215
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发表时间：
2004-01-30
影响因子：
0.4
通讯作者：
Yasuda, K
中科院分区：
文献类型：
--
作者：
Moriguchi, H;Takahashi, K;Yasuda, K
我们已经开发了一种新的方法，用于在玻璃载玻片上的柔性琼脂糖微室阵列上培养神经细胞。琼脂糖微室是在琼脂糖层表面形成的微米级空腔，通过成型50 μ m高的方形/圆形微铸体厚SU-8光刻胶。此外，采用光热蚀刻法重新排列琼脂糖微室的形状，其中我们使用红外（1064 nm）聚焦激光束作为热源，在加热点熔化并去除部分琼脂糖凝胶。利用光热蚀刻法，我们还可以在微室之间制造狭窄的隧道状通道。当神经细胞在琼脂微室阵列芯片上培养时，两个相邻微室中的神经细胞在培养48小时后通过光热蚀刻通道连接。这些结果表明，琼脂糖微室阵列与光热蚀刻方法相结合，在单细胞培养和神经细胞测量的下一阶段具有潜力，例如在培养过程中实时控制细胞相互作用。(C) 2003 Wiley期刊有限公司
We have developed a novel method for on-chip cultivation of neural cells in a flexible agarose-microchamber array on a glass slide. The agarose microchamber is a micrometer-order cavity constructed on the surface of an agarose layer by molding a 50-mum-high square/circular micro-cast of thick SU-8 photoresist. In addition, the shape of the agarose microchamber was rearranged by using the photothermal etching method, in which we used an infrared (1064-nm) focused laser beam as the heat source to melt and remove a portion of agarose gel at the heating spot. With the photothermal etching method, we can also manufacture narrow tunnel-shaped channels between microchambers. When nerve cells were cultured on the agar-microchamber array chip, the nerve cells in two adjacent microchambers connected through the photothermal-etched channel after 48 hours of cultivation. Those results suggest the potential of an agarose-microchamber array integrated with the photothermal etching method for the next stage of single cell cultivation and measurement of nerve cells, such as real-time control of cell interactions during cultivation. (C) 2003 Wiley Periodicals, Inc.