Effects of lymphokines and mitogens on a histamine derivative-induced intracellular calcium mobilization and inositol phosphate production.
Effects of lymphokines and mitogens on a histamine derivative-induced intracellular calcium mobilization and inositol phosphate production.
复制标题
淋巴因子和有丝分裂原对组胺衍生物诱导的细胞内钙动员和磷酸肌醇产生的影响。
DOI：
10.1016/0006-2952(94)90086-8
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发表时间：
1994
影响因子：
5.8
通讯作者：
Khan,MM
中科院分区：
文献类型：
--
作者：
Qiu,R;Melmon,KL;Khan,MM
组胺三氟甲基甲酰衍生物（HTMT）是一种新型免疫抑制剂，可刺激淋巴细胞中的H1、h2和HTMT受体。HTMT受体不同于经典的H2、H2or h3受体。HTMT受体的刺激导致人外周血淋巴细胞内钙（[Ca2+]i）和肌醇磷酸（IP）浓度的增加。在本研究中，我们研究了淋巴因子[白介素-4 (IL-4)，白介素-2 (IL-2)]和其他药物[脂多糖（LPS）， phorbol 12-肉豆酸酯13-乙酸酯（PMA）]对htmt诱导的非莲座细胞Ca2+和IP反应的影响。当细胞用IL-4预处理时，HTMT引起增强的[Ca2+] iip反应。IL-4的作用呈浓度依赖性，作用48小时后达到最大。蛋白质合成抑制剂，而非RNA合成抑制剂，阻断了IL-4对htmt诱导反应的作用。LPS比IL-4更能增强HTMT诱导的Ca2+动员。然而，LPS的作用不受蛋白质合成或RNA转录抑制剂的影响。这表明LPS对HTMT反应的作用可能与IL-4不同。IL-2和PMA不影响htmt诱导的[Ca2+]陆地IP反应。IL-4和LPS的作用是激动剂特异性的。它们不影响PAF诱导的Ca2+动员。这些数据表明，对HTMT的反应可受IL-4和LPS的调控。虽然这些受体在体内的重要性尚不清楚，但受体可能是免疫和/或炎症调节的一个贡献者。
Histamine trifluoromethyl-toluidide derivative (HTMT), a novel imrnunosuppressive agent, stimulates H1, H2and HTMT receptors in lymphocytes. HTMT receptors are different from the classical H2, H2or H3receptors. Stimulation of HTMT receptors results in increased intracellular concentrations of calcium ([Ca2+]i) and inositol phosphate (IP) in human peripheral blood lymphocytes. In the present study, we investigated the effects of lymphokines [interleukin-4 (IL-4), interleukin-2 (IL-2)] and other pharmacologic agents [lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate (PMA)] on HTMT-induced Ca2+and IP responses in non-rosetted cells. HTMT caused enhanced [Ca2+]iand IP responses when the cells were pretreated with IL-4. The effects of IL-4 were concentration dependent and became maximal after the cells were incubated with IL-4 for 48 hr. Inhibitors of protein synthesis, but not of RNA synthesis, blocked the effects of IL-4 on HTMT-induced responses. LPS was more potent than IL-4 in augmenting Ca2+mobilization induced by HTMT. However, the effects of LPS were not altered by inhibitors of either protein synthesis or RNA transcription. This indicated that LPS may act differently than IL-4 on the HTMT response. IL-2 and PMA did not affect HTMT-induced [Ca2+]iand IP responses. The effects of IL-4 and LPS were agonist specific. They did not affect the Ca2+mobilization induced by PAF. The data indicate that the response to HTMT can be regulated by IL-4 and LPS. Although thein vivoimportance of these receptors is not yet clear, the receptor is likely a contributor to immune and/or inflammatory regulation.