Biochar reduced soil nitrous oxide emissions through suppressing fungal denitrification and affecting fungal community assembly in a subtropical tea plantation
Biochar reduced soil nitrous oxide emissions through suppressing fungal denitrification and affecting fungal community assembly in a subtropical tea plantation
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生物炭通过抑制亚热带茶园真菌反硝化和影响真菌群落组装来减少土壤一氧化二氮排放
DOI：
10.1016/j.agee.2021.107784
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发表时间：
2022-03-01
影响因子：
6.6
通讯作者：
Zou, Jianwen
中科院分区：
文献类型：
--
作者：
Ji, Cheng;Han, Zhaoqiang;Zou, Jianwen
生物炭改良剂已被证明可以减少茶园酸性土壤的氧化亚氮（N2 O）排放。鉴于土壤细菌和真菌的发酵都能产生N2 O，它们对生物炭改良后N2 O排放减少的相对贡献尚不清楚。在这里，我们研究了土壤样品从亚热带种植园，已收到2年的生物炭修正案。真菌和细菌对N2 O产生的相对贡献的测量被采取了由底物诱导的呼吸方法实施与选择性抑制剂的加入。通过q-PCR对总真菌、细菌和关键N2 O相关细菌基因的丰度进行定量，并通过18 S rRNA扩增子测序分析真菌群落的组成。结果表明，在施氮的酸性土壤中，真菌对N2 O的贡献率（52%）大于细菌（18%）。生物炭的修订显着降低真菌丰度和真菌的贡献N2 O的生产（由28%）。相比之下，生物炭改良剂显著增加了N2 O相关细菌的丰度（例如，氨氧化细菌（AOB）、nirS、nosZ）。结构方程模型（SEM）表明，生物炭通过提高土壤pH值和改变真菌群落组成，降低了真菌对N2 O产生的贡献。我们的研究结果强调，减少N2 O排放量可以归因于刺激N2 O消耗过程（驱动N2 O消耗细菌编码的nosZ基因）和抑制真菌优势在酸性土壤茶园。本研究对生物炭对土壤微生物介导的N2 O产生过程的调控作用提供了较为全面的见解。nbsp;
Biochar amendment has been shown to reduce nitrous oxide (N2O) emissions from acidic soils in tea plantations. Given that both soil bacterial and fungal denitrifications can produce N2O, their relative contributions to the decrease in N2O emissions following biochar amendment remain unclear. Here, we examined soils sampled from a subtropical tea plantation that had received 2 years of biochar amendment. Measurements of the relative contributions of fungi and bacteria to N2O production were taken by the substrate-induced respiration method implemented with the addition of selective inhibitors. The abundances of total fungi, bacteria, and key N2O-related bacterial genes were quantified by q-PCR, and the composition of the fungal community was analyzed by 18S rRNA amplicon sequencing. The results showed that the contribution of fungi toN(2)O production (52%) was greater than that of bacteria (18%) for the N-applied acidic soils. Biochar amendment significantly decreased the fungal abundances and the fungal contribution to N2O production (by 28%). In contrast, biochar amendment significantly increased the abundances of N2O-related bacteria (e.g., ammonia-oxidizing bacteria (AOB), nirS, nosZ). Structural equation models (SEMs) revealed that biochar decreased the fungal contribution to N2O production through enhancing the soil pH and shifting the fungal community composition. Our results highlight that the decreased N2O & nbsp;emissions could be ascribed to the stimulated N2O consumption process (driven by N2O-consuming bacteria encoded by the nosZ gene) and suppressed fungal dominance in acidic soils from tea plantations. This study presents relatively comprehensive insights into the regulatory roles of biochar on soil microbe mediated N2O production processes.& nbsp;& nbsp;