Peanut Agglutinin
Peanut Agglutinin
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花生凝集素
DOI：
10.1111/j.1365-3083.1979.tb03067.x
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发表时间：
1979
影响因子：
3.7
通讯作者：
P. Niaudet
中科院分区：
文献类型：
--
作者：
J. London;Perrot Jy;S. Berrih;L. Laroche;P. Niaudet
花生凝集素 (PNA) 此前已被证明是小鼠早期 T 亚群的“标记”。我们研究了它是否也可以用于人类单核细胞的研究。 50-60% 的人类胸腺细胞具有 PNA 结合位点。当在不连续的 Ficoll 梯度上分离时，PNA 阳性胸腺细胞优先出现在与免疫无能细胞相对应的层中。在外周血中，只有 5% 的单核细胞呈 PNA 阳性，我们已经证明这些细胞是单核细胞。在扁桃体中发现了 13% 的 PNA+ 细胞，它们大部分是淋巴细胞。因此，PNA 是胸腺和扁桃体中某些 T 细胞亚群的标记。
Peanut agglutinin (PNA) has previously been shown to be a ‘marker’ for early T subpopulations in mice. We have investigaied whether it could also be used in the study of human mononuclear cells. 50–60% of human thymocytes have binding sites for PNA. When separated on a discontinuous Ficoll gradient, the PNA‐positive thymocytes are found preferentially in the layers corresponding to the immunoincompetent cells. In the peripheral blood only 5% of the mononuclear cells are PNA‐positive and we have shown that these cells are monocytes. In tonsils 13% of PNA+ cells are found and they are mostly lymphocytes. Thus, PNA is a marker for some T cell subsets present in the thymus and tonsils.