Effects of host genotype against the expression of spiroplasma-induced male killing in Drosophila melanogaster
Effects of host genotype against the expression of spiroplasma-induced male killing in Drosophila melanogaster
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DOI：
10.1038/hdy.2009.14
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发表时间：
2009-05-01
期刊：
影响因子：
3.8
通讯作者：
Fukatsu, T.
中科院分区：
文献类型：
--
作者：
Kageyama, D.;Anbutsu, H.;Fukatsu, T.
人们越来越关注母系遗传的微生物，它们能够为了自身利益而操纵宿主的繁殖。尽管一些研究表明宿主基因型可以影响操纵的强度，但其潜在的遗传基础却知之甚少。在这里，我们检查了各种野生型黑腹果蝇种群中螺原体诱导的雄性杀伤强度，以阐明导致雄性杀伤强度变化的宿主因素的遗传基础。在通过交配实验（即核渗入）检查的 10 个品系中，包括 Oregon-R 和 Canton-S 在内的 8 个品系被发现具有允许强烈表达雄性杀伤的核因子。相反，Sevelen和Hikone品系的核因子部分抑制或显着延迟雄性杀戮的表达。这些结果通过显微注射的螺旋原体感染跨果蝇系的人工转移实验得到证实。一系列的交配实验表明，对抗雄性杀戮的核因子主要位于Sevelen的常染色体上和Hikone的X染色体上。在这两个系中，抑制因子被推断为具有显性效应的母性行为。 Sevelen 和 Hikone 的核因子几乎不影响生殖活跃的幼虫中的螺原体密度，这表明这些抑制因子可能直接作用于雄性杀伤表达，而不是通过抑制细菌增殖。遗传 (2009) 102, 475-482; doi：10.1038/hdy.2009.14； 2009 年 2 月 18 日在线发布
Increasing attention has been paid to the maternally inherited microbes that are capable of manipulating the reproduction of their hosts for their own benefit. Although several studies have revealed that the host genotype can affect the intensity of the manipulation, the underlying genetic basis is poorly understood. Here, we examined the intensity of spiroplasma-induced male killing in various wild-type stocks of Drosophila melanogaster to clarify the genetic basis of the host factors responsible for the variation in the male-killing intensity. Among ten lines examined by mating experiments (that is, nuclear introgression), eight lines including Oregon-R and Canton-S were found to have nuclear factors that allowed strong expression of male killing. In contrast, the nuclear factors of the lines Sevelen and Hikone partially suppressed or remarkably retarded the expression of male killing. These results were confirmed by artificial transfer experiments of spiroplasma infection across the fly lines by means of microinjection. A series of mating experiments revealed that the nuclear factors acting against male killing were mainly located on autosomes in Sevelen and on the X chromosome in Hikone. In both lines, the suppressors were inferred to act maternally with a dominant effect. The nuclear factors of Sevelen and Hikone scarcely affected spiroplasma densities in reproductively active young insects, suggesting that the suppressors may act on the male-killing expression directly rather than through suppressing bacterial proliferation. Heredity (2009) 102, 475-482; doi:10.1038/hdy.2009.14; published online 18 February 2009