TN10-ENCODED TET-REPRESSOR CAN REGULATE AN OPERATOR-CONTAINING PLANT PROMOTER
TN10-ENCODED TET-REPRESSOR CAN REGULATE AN OPERATOR-CONTAINING PLANT PROMOTER
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DOI：
10.1073/pnas.85.5.1394
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发表时间：
1988-03-01
影响因子：
11.1
通讯作者：
QUAIL, PH
中科院分区：
文献类型：
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作者：
GATZ, C;QUAIL, PH
Tn 10编码的泰特抑制子-操纵子系统用于调节花椰菜花叶病毒（CaMV）35 S启动子的转录。通过使用电场介导的基因转移（“电穿孔”）进入烟草原生质体，在瞬时测定系统中监测表达。在真核转录信号的控制下在植物细胞中表达的泰特阻遏物，当两个泰特操纵子位于“TATA”盒的侧翼时，阻断CaMV 35 S启动子氯霉素乙酰转移酶（cat）融合基因的转录。在诱导剂四环素存在下，表达恢复至完全活性。操纵基因位于转录起始位点下游21个碱基对的位置在阻遏物存在下不会显著影响转录。这些实验表明，原核调节蛋白可以在植物细胞中发挥作用。泰特阻遏子-操纵子复合物可用于在植物发育的不同阶段特异性地诱导转移的基因。
The Tn10-encoded tet repressor-operator system was used to regulate transcription from the cauliflower mosaic virus (CaMV) 35S promoter. Expression was monitored in a transient assay system by using electric field-mediated gene transfer ("electroporation") into tobacco protoplasts. The tet repressor, being expressed in the plant cells under the control of eukaryotic transcription signals, blocks transcription of a CaMV 35S promoter chloramphenicol acetyltransferase (cat) fusion gene when the two tet operators flank the "TATA" box. In the presence of the inducer tetracycline, expression is restored to full activity. Location of the operators 21 base pairs downstream of the transcription start site does not significantly affect transcription in the presence of the repressor. These experiments show that a prokaryotic regulatory protein can function in plant cells. The tet repressor-operator complex may be useful for specifically inducing transferred genes at different stages of plant development.