Intracellular pH regulation by a Na+/H+ exchanger in cultured bovine trabecular cells.
Intracellular pH regulation by a Na+/H+ exchanger in cultured bovine trabecular cells.
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培养牛小梁细胞中 Na /H 交换剂对细胞内 pH 值的调节。
DOI：
10.1111/j.1755-3768.1992.tb04886.x
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发表时间：
1992
影响因子：
3.4
通讯作者：
Green,K
中科院分区：
文献类型：
--
作者：
Chu,TC;Keith,C;Green,K
使用视频成像技术和pH敏感性细胞内荧光染料BCECF测量培养的牛小梁细胞的细胞内pH（pHi）。在pH 7.4的富碳酸氢盐林格氏液中，pH为7.29 ± 0.03（± SEM，n = 12个单层，120个细胞采样）。暴露于20 mM NH 4Cl立即碱化pH：在恢复至静息水平之前，用富含Na+的溶液置换酸化pH。当用低Na+溶液代替NH 4Cl时，酸化持续，但在富Na+溶液后发生pH恢复。在pH 6.8下，pH值为7.11 ± 0.02（n = 2个单层，20个细胞），在pH 8.0下，pH值为7.72 ±0.03（n = 2个单层，20个细胞）。阿米洛利（1 mM）酸化pHi，但DIDS（1 mM）处理、无HCO 3 −条件、1 mM哇巴因、50 mM K+和2 mM BaCl 2未能改变pHi。过氧化氢（1 mM）酸化pH值，但50 μM时无变化。小梁细胞具有类似于其他细胞类型的Na+/H+交换器。
Intracellular pH (pHi) of cultured bovine trabecular cells was measured using video‐imaging techniques with a pH‐sensitive intracellular fluorescent dye, BCECF. In bicarbonate‐rich Ringer at pH 7.4, pHiwas 7.29 ± 0.03 (± SEM, n = 12 monolayers, 120 cells sampled). Exposure to 20 mM NH4Cl immediately alkalinized pHi: replacement with a Na+‐rich solution acidified pHibefore recovery to resting levels. When NH4Cl was replaced by a low Na+solution, acidification was sustained but pHirecovery occurred after Na+‐rich solution. A pHiof 7.11 ± 0.02 (n = 2 monolayers, 20 cells) occurred in pH 6.8 and pHiwas 7.72 ±0.03 (n = 2 monolayers, 20 cells) in pH 8.0. Amiloride (1 mM) acidified pHibut DIDS (1 mM) treatment, HCO3−‐free condition, 1 mM ouabain, 50 mM K+, and 2 mM BaCl2failed to change pHi. Hydrogen peroxide (1 mM) acidified pHibut no change occurred with 50 μM. Trabecular cells possess an Na+/H+exchanger similar to that in other cell types.