Effect of exercise on serum concentration of cartilage oligomeric matrix protein in Thoroughbreds
Effect of exercise on serum concentration of cartilage oligomeric matrix protein in Thoroughbreds
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DOI：
10.2460/ajvr.68.2.134
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发表时间：
2007-02-01
影响因子：
1
通讯作者：
Fujiki, Makoto
中科院分区：
文献类型：
--
作者：
Helal, Ibrahim E.;Misumi, Kazuhiro;Fujiki, Makoto
目的：探讨运动对马血清软骨寡聚基质蛋白（COMP）浓度的影响。动物——实验1 15匹马，实验2 27匹马。在实验1中，15只没有骨科疾病的纯种马接受了标准化的运动方案。记录跑步速度和心率（HR），并在单次运动前（基线）、1小时、5小时和24小时采集血液样本。在实验2中，27匹马接受了9个阶段的训练计划，每个阶段包括4到8个连续的日常训练，然后休息一天。在每个阶段的第一次和最后一次日常训练之前立即采集血液样本。用抗马COMP单克隆抗体（14G4）抑制ELISA法测定血清COMP浓度。结果：实验1中，运动后1和5小时，马血清COMP平均浓度显著高于基线水平，运动后24小时恢复到基线水平。平均血清基线COMP浓度随着最大心率和心率为200次/分时跑步速度的增加而增加，在第三和第四次运动试验中显著高于第一次运动试验。在实验2中，从第3阶段开始，每个阶段最后训练的平均基线COMP浓度都显著高于第一次训练时的浓度。结论与临床相关性：运动后血清COMP浓度发生显著变化。运动可以促进COMP进入循环，并改变COMP的基础周转率。
Objective-To evaluate changes in serum cartilage oligomeric matrix protein (COMP) concentrations in response to exercise in horses.Animals-15 horses in experiment 1 and 27 horses in experiment 2.Procedures-In experiment 1, 15 Thoroughbreds free of orthopedic disease underwent a standardized exercise protocol. Running velocity and heart rate (HR) were recorded, and blood samples were collected immediately before (baseline) and 1, 5, and 24 hours after a single episode of exercise. In experiment 2, 27 horses underwent 9 stages of a training program in which each stage consisted of 4 to 8 consecutive daily workouts followed by a rest day. Blood samples were collected immediately before the first and final daily workouts in each stage. Serum COMP concentrations were measured via inhibition ELISA with a monoclonal antibody (14G4) against equine COMPResults-In experiment 1, mean serum COMP concentration was significantly higher than baseline 1 and 5 hours after exercise and returned to baseline concentrations 24 hours after exercise. Mean serum baseline COMP concentration increased as the velocity of running at maximum HR and at an HR of 200 beats/min increased, being significantly higher during the third and fourth exercise tests than during the first. In experiment 2, mean baseline COMP concentration at the final workout of each stage was significantly higher than that at the first workout, beginning with stage 3.Conclusions and Clinical Relevance-Serum COMP concentrations changed significantly in response to exercise. Exercise may enhance movement of COMP into the circulation as well as change the basal turnover rate of COMP.