ANXA5 level is linked to in vitro and in vivo tumor malignancy and lymphatic metastasis of murine hepatocarcinoma cell
ANXA5 level is linked to in vitro and in vivo tumor malignancy and lymphatic metastasis of murine hepatocarcinoma cell
复制标题
ANXA5水平与小鼠肝癌细胞的体外和体内肿瘤恶性肿瘤和淋巴转移有关
DOI：
10.2217/fon.15.289
复制
发表时间：
2016-01-01
期刊：
影响因子：
3.3
通讯作者：
Sun, Ming-Zhong
中科院分区：
文献类型：
--
作者：
Peng, Boya;Liu, Shuqing;Sun, Ming-Zhong
目的：研究 ANXA5 过表达对小鼠 Hca-P 细胞体外和体内恶性肿瘤的影响。材料和方法：使用具有低淋巴结转移（LNM）潜力的Hca-P作为细胞模型。对体外 Hca-P 行为进行 TEM、CCK-8 和 Boyden transwell 测定。建立Hca-P移植小鼠模型进行体内实验。结果：获得ANXA5过表达的单克隆Anxa5-Hca-P-1、Anxa5-Hca-P-2和Anxa5-Hca-P-3细胞。 ANXA5上调改变Hca-P细胞的增殖、形态和粗面内质网，增强Hca-P的体外迁移和侵袭，促进Anxa5-Hca-P-3移植小鼠的体内恶性程度和LNM率。结论：ANXA5过表达作为恶性肿瘤和淋巴转移的潜在指标，可增加Hca-P细胞的体外迁移和侵袭，促进Hca-P移植小鼠的体内恶性肿瘤、LNM发生率和水平。
Aim: To investigate ANXA5 overexpression on in vitro and in vivo malignancies of murine Hca-P cells. Materials & methods: Hca-P with low lymph node metastasis (LNM) potential was used as cell model. TEM, CCK-8 and Boyden transwell assays were performed for in vitro Hca-P behaviors. Hca-P-transplanted mouse model was established for in vivo experiment. Results: ANXA5-overexpressing monoclonal Anxa5-Hca-P-1, Anxa5-Hca-P-2 and Anxa5-Hca-P-3 cells were obtained. ANXA5 upregulation alters the proliferation, morphology and rough endoplasmic reticulum of Hca-P cells, enhances in vitro migration and invasions of Hca-P, promotes in vivo malignant degree and LNM rate of Anxa5-Hca-P-3-transplanted mice. Conclusion: As a potential indicator for malignancy and lymphatic metastasis, ANXA5 overexpression increases in vitro migration and invasion of Hca-P cell, promotes in vivo malignancy, LNM rate and level of Hca-P-transplanted mice.