Secretion of a new spherical body protein of Babesia bovis into the cytoplasm of infected erythrocytes
Secretion of a new spherical body protein of Babesia bovis into the cytoplasm of infected erythrocytes
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DOI：
10.1016/j.molbiopara.2011.02.006
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发表时间：
2011-07-01
影响因子：
1.5
通讯作者：
Igarashi, Ikuo
中科院分区：
文献类型：
--
作者：
Terkawi, Mohamad Alaa;Seuseu, Faasoa Junior;Igarashi, Ikuo
牛巴氏杆菌球蛋白4（BbSBP-4）的cDNA与其他顶复门蛋白或以前报道的B没有明显的同源性。牛球状体蛋白在本研究中，我们进一步研究了BbSBP-4的分子特征，包括BbSBP-4的表达和细胞定位。抗rBbSBP-4小鼠血清与41-kDa天然蛋白B特异性反应。Western blot分析。免疫电镜检查证实BbSBP-4定位在球形体，但不是在核，棒状体，和微丝。有趣的是，发现该蛋白质不仅定位于B的球体中。牛，而且在寄生虫发育的后期感染的红细胞（iRBC）的细胞质中。激光共聚焦显微镜观察和Western blot分析表明，BbSBP-4在iRBC细胞质和培养B细胞上清液中的积累增加。牛在寄生虫的后期发育阶段。这些结果表明，BbSBP-4在感染红细胞破裂之前的寄生虫发育后期从球形体分泌到iRBC的细胞质中。综上所述，BbSBP-4可能作为分泌蛋白在B的细胞内发育和/或存活中发挥重要作用。牛(C)2011 Elsevier B. V.保留所有权利。
A cDNA encoding a new Babesia bovis spherical body protein 4 (BbSBP-4) was reported to have no significant homology to other apicomplexan proteins or previously reported B. bovis spherical body proteins. In the present study, we further examined the molecular characteristics of BbSBP-4 including the expression and cellular localization of the BbSBP-4. An anti-rBbSBP-4 mouse serum specifically reacted to a 41-kDa native protein B. bovis in Western blot analysis. The immunoelectron microscopic examination confirmed the localization of BbSBP-4 in spherical bodies, but not in the nucleus, rhoptries, and micronemes. Interestingly, the protein was found to be localized not only in the spherical body of B. bovis but also in the cytoplasm of infected erythrocytes (iRBC) at the later stage of parasite development. The confocal laser microscopic examination and Western blot analysis demonstrated the increased accumulation of BbSBP-4 in the cytoplasm of iRBC and in the supernatant of cultivated B. bovis during the late developmental stage of the parasite. These results suggest that BbSBP-4 was secreted from spherical body into cytoplasm of iRBC during the late developmental stage of the parasite before the rupture of infected RBC. Taken together, BbSBP-4 might play an important role as a secreted protein in the intracellular development and/or survival of B. bovis. (C) 2011 Elsevier B.V. All rights reserved.