Polarized light microscopy in reproductive and developmental biology.
Polarized light microscopy in reproductive and developmental biology.
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DOI：
10.1002/mrd.22221
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发表时间：
2015-07
影响因子：
2.5
通讯作者：
Oldenbourg R
中科院分区：
文献类型：
--
作者：
Koike-Tani M;Tani T;Mehta SB;Verma A;Oldenbourg R
偏振光显微镜揭示了活细胞、组织和整个生物体内天然分子结构的取向顺序。它是一种强大的工具，用于监测和分析适合显微镜观察的生物体的早期发育阶段。在这篇文章中，我们简要地讨论了特定于传统的偏光显微镜和一些历史上重要的意见：染色体包装在精子头，第一次受精卵分裂的海胆，和分化发起的第一次不对称细胞分裂的沙美元。然后，我们介绍了LC-PolScope，并描述了其用于测量活细胞和组织中的双折射和偏振荧光。应用范围从小鼠卵母细胞去核到分析水虻顶体的偏振荧光。最后，我们对发育中的鸡脑的双折射的新结果，我们分析了12-20天的发育阶段之间。
The polarized light microscope reveals orientational order in native molecular structures inside living cells, tissues, and whole organisms. It is a powerful tool used to monitor and analyze the early developmental stages of organisms that lend themselves to microscopic observations. In this article, we briefly discuss the components specific to a traditional polarizing microscope and some historically important observations on: chromosome packing in the sperm head, the first zygote division of the sea urchin, and differentiation initiated by the first asymmetric cell division in the sand dollar. We then introduce the LC-PolScope and describe its use for measuring birefringence and polarized fluorescence in living cells and tissues. Applications range from the enucleation of mouse oocytes to analyzing the polarized fluorescence of the water strider acrosome. We end with new results on the birefringence of the developing chick brain, which we analyzed between developmental stages of days 12–20.