Age-related change in numbers of other interstitial cells in testes of adult men: evidence bearing on the fate of Leydig cells lost with increasing age.
Age-related change in numbers of other interstitial cells in testes of adult men: evidence bearing on the fate of Leydig cells lost with increasing age.
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DOI：
10.1095/biolreprod33.1.259
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发表时间：
1985-08
影响因子：
3.6
通讯作者：
W. Neaves;L. Johnson;C. S. Petty
中科院分区：
文献类型：
--
作者：
W. Neaves;L. Johnson;C. S. Petty
成年人睾丸间质细胞的数量随着年龄的增长而下降，但这些细胞是否通过转化为另一种细胞类型或通过死亡和溶解而消失尚未解决。这个问题在30名20至76岁之间的男性中得到了解决，他们被认为经历了与年龄相关的Leydig细胞严重丢失。如果Leydig细胞的丢失是由于转化为另一种细胞类型，那么这些男性的其他睾丸间质细胞应该随着年龄的增长而增加。在因创伤或心脏病发作而猝死后15小时内，用戊二醛灌注尸检获得的睾丸。其他间质细胞的数量通过定量组织学估计睾丸实质中其他间质细胞核所占的比例来确定。其他间质细胞核随年龄增长而显著下降（rho =-0.41，P <0.05）。与15名年轻男性相比，15名50岁或50岁以上男性的其他间质细胞核平均数量显著减少（4.6亿+/-3400万vs. 6.09亿+/-4300万; P <0.05）。Leydig细胞数量减少的个体没有其他间质细胞数量增加的趋势。这些研究结果反对间质细胞在老年睾丸中作为去分化的间充质成分的持续存在。相反，对这些男性睾丸的光镜和电镜观察显示了Leydig细胞变性和溶解的证据。
The number of Leydig cells in the adult human testis declines as a function of increasing age, but whether these cells disappear by transforming into another cell type or by undergoing death and dissolution has not been resolved. This question was addressed in 30 men between 20 and 76 years of age who were known as a group to have experienced significant age-related loss of Leydig cells. If the loss of Leydig cells resulted from transformation into another cell type, other testicular interstitial cells in these men should have increased with age. Testes obtained at autopsy were perfused with glutaraldehyde less than 15 h after sudden death due to trauma or heart attack. Numbers of other interstitial cells were determined by quantitative histometric estimation of the proportion of testicular parenchyma occupied by other interstitial cell nuclei of measured average volume. Other interstitial cell nuclei declined significantly with advancing age (rho = -0.41, P less than 0.05). Mean number of other interstitial cell nuclei per individual was significantly reduced in the 15 men 50 yr old or older compared to the 15 younger men (460 +/- 34 million vs. 609 +/- 43 million; P less than 0.05). There was no tendency for individuals with reduced numbers of Leydig cells to have increased numbers of other interstitial cells. These findings argued against the persistence of Leydig cells in aged testes as dedifferentiated mesenchymal elements. Instead, light and electron microscopic observation of testes from these men revealed evidence of Leydig cell degeneration and dissolution.