Calibration of the TonoLab Tonometer in Mice with Spontaneous or Experimental Glaucoma
Calibration of the TonoLab Tonometer in Mice with Spontaneous or Experimental Glaucoma
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DOI：
10.1167/iovs.10-5556
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发表时间：
2011-02-01
影响因子：
4.4
通讯作者：
Quigley, Harry A.
中科院分区：
文献类型：
--
作者：
Pease, Mary E.;Cone, Frances E.;Quigley, Harry A.
目的.测量TonoLab（TioLat，赫尔辛基，芬兰）眼压计在自发或诱导实验性青光眼小鼠中的准确性。通过注射聚苯乙烯珠和粘弹性材料，在32只小鼠的一只眼中诱导慢性眼内压（IOP）升高。3 - 6周后，对眼睛进行插管，并将压力计设置为10、20、30、40或50 mm Hg。小鼠为8周龄和8月龄的C57BL/6、8周龄的DBA/2J和8周龄的CD 1。TonoLab校准也在五只患有自发性青光眼的老年DBA/2J小鼠上进行了测试。在多变量线性回归模型中评价TonoLab阅读值与眼压测量值的关系，模型中以眼轴长度、IOP病史和小鼠品系作为自变量。在所有小鼠中，TonoLab与测压IOP之间的关系的斜率为0.998，截距为2.3 mm Hg（单变量回归中的校正R = 0.86）。无论是珠诱导青光眼的小鼠还是自发性青光眼的小鼠（老年DBA/2J小鼠），在TonoLab和眼压计IOP之间具有良好的相关性方面，与对照动物都没有显著差异。无论是青光眼还是对照组，小鼠眼睛越长越宽，眼压测量的IOP越高（多变量回归，校正R-2 = 0.90，P <0.0001）。在三种小鼠品系之间：CD 1、C57BL/6和DBA/2J，以及8周龄和8月龄的C57BL/6小鼠之间，眼压测量准确性没有差异（多变量回归，P = 0.32）。TonoLab准确地反映了正常小鼠和实验性或自发性青光眼小鼠眼睛的IOP，没有检测到年龄的影响。（Invest Ophthalmol维斯科学。2011; 52：858 - 864）DOI：10.1167/iovs.10 - 5556
PURPOSE. To measure the accuracy of TonoLab (TioLat, Helsinki, Finland) tonometry in mice with spontaneous or induced experimental glaucoma.METHODS. Chronic intraocular pressure (IOP) elevation was induced in one eye of 32 mice by injection of polystyrene beads and viscoelastic material. Three to 6 weeks later, the eyes were cannulated and manometrically set to 10, 20, 30, 40, or 50 mm Hg. The mice were 8-week and 8-month-old C57BL/6, 8-week-old DBA/2J, and 8-week-old CD1. The TonoLab calibration was also tested on five aged DBA/2J mice with spontaneous glaucoma. The relation of the TonoLab reading to manometric IOP was evaluated in multivariate linear regression models with axial length, IOP history, and mouse strain as independent variables.RESULTS. The slope of the relationship between TonoLab and manometric IOP in all the mice was 0.998, with an intercept of 2.3 mm Hg (adjusted R in univariate regression = 0.86). Neither the mice with bead-induced glaucoma nor those with spontaneous glaucoma (older DBA/2J mice) differed significantly from the control animals in having an excellent correlation between TonoLab and manometer IOP. Longer and wider mouse eyes had slightly higher tonometrically measured IOP, whether glaucomatous or control (multivariate regression, adjusted R-2 = 0.90, P < 0.0001). There was no difference in tonometric accuracy among the three mouse strains: CD1, C57BL/6, and DBA/2J, nor between 8-week and 8-month-old C57BL/6 mice (multivariate regression, P = 0.32).CONCLUSIONS. The TonoLab accurately reflects IOP in both normal mice and in eyes of mice with experimental or spontaneous glaucoma, with no detectable effect of age. (Invest Ophthalmol Vis Sci. 2011;52:858-864) DOI:10.1167/iovs.10-5556