The optimal anatomic sites for sampling heterosexual men for human papillomavirus (HPV) detection: The HPV detection in men study
The optimal anatomic sites for sampling heterosexual men for human papillomavirus (HPV) detection: The HPV detection in men study
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DOI：
10.1086/521629
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发表时间：
2007-10-15
影响因子：
6.4
通讯作者：
Harris, Robin B.
中科院分区：
文献类型：
--
作者：
Giuliano, Anna R.;Nielson, Carrie M.;Harris, Robin B.
背景人乳头瘤病毒（HPV）感染的男性有助于感染和宫颈疾病的妇女以及疾病的男性。本研究旨在确定异性恋男性HPV检测的最佳解剖部位。从2003年到2006年，在463名男性中进行了HPV感染的横断面研究。采集尿道、阴茎头/冠状沟、阴茎干/包皮、阴囊、肛周、肛管、精液和尿液样本。通过聚合酶链反应和基因分型分析样本的样本充分性和HPV DNA。为了确定估计HPV患病率的最佳位点，计算了位点特异性患病率，并与总体患病率进行了比较。排除研究中心和研究中心组合，直到重新计算的患病率比总体患病率降低< 5%。HPV总感染率为65.4%。HPV在阴茎干的检出率最高（全队列为49.9%，子队列为47.9%），其次是阴茎头/冠状沟（35.8%和32.8%）和阴囊（34.2%和32.8%）。尿道（10.1%和10.2%）和精液（5.3%和4.8%）样本的检出率最低。排除尿道、精液和肛周、阴囊或肛门样本导致患病率降低< 5%。至少，应该对异性恋男性的阴茎干和阴茎头/冠状沟进行采样。阴囊，肛周或肛门样本也应该包括最佳HPV检测。
Background. Human papillomavirus (HPV) infection in men contributes to infection and cervical disease in women as well as to disease in men. This study aimed to determine the optimal anatomic site(s) for HPV detection in heterosexual men.Methods. A cross-sectional study of HPV infection was conducted in 463 men from 2003 to 2006. Urethral, glans penis/coronal sulcus, penile shaft/prepuce, scrotal, perianal, anal canal, semen, and urine samples were obtained. Samples were analyzed for sample adequacy and HPV DNA by polymerase chain reaction and genotyping. To determine the optimal sites for estimating HPV prevalence, site-specific prevalences were calculated and compared with the overall prevalence. Sites and combinations of sites were excluded until a recalculated prevalence was reduced by < 5% from the overall prevalence.Results. The overall prevalence of HPV was 65.4%. HPV detection was highest at the penile shaft (49.9% for the full cohort and 47.9% for the subcohort of men with complete sampling), followed by the glans penis/coronal sulcus (35.8% and 32.8%) and scrotum (34.2% and 32.8%). Detection was lowest in urethra (10.1% and 10.2%) and semen (5.3% and 4.8%) samples. Exclusion of urethra, semen, and either perianal, scrotal, or anal samples resulted in a < 5% reduction in prevalence.Conclusions. At a minimum, the penile shaft and the glans penis/coronal sulcus should be sampled in heterosexual men. A scrotal, perianal, or anal sample should also be included for optimal HPV detection.