A sensitized chemiluminescence system based on the reaction between Ni(IV) complex and luminol: applications to the determinations of β2-agonist drugs
A sensitized chemiluminescence system based on the reaction between Ni(IV) complex and luminol: applications to the determinations of β2-agonist drugs
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基于 Ni(IV) 配合物和鲁米诺反应的敏化化学发光系统：在β2 激动剂药物测定中的应用
DOI：
10.1007/s00706-016-1690-1
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发表时间：
2016-10-01
影响因子：
1.8
通讯作者：
Shi，Hongmei
中科院分区：
文献类型：
--
作者：
Diao，Yajie;Dong，Xiao;Shi，Hongmei
建立了一种新的、简单而有效的化学发光体系，用于β2-受体激动剂莱克多巴胺和非诺特罗的测定。该系统是基于在碱性介质中的Ni（IV）配合物与鲁米诺的化学发光反应，而莱克多巴胺或非诺特罗可以显着增强化学发光强度。在最佳条件下，莱克多巴胺和非诺特罗的化学发光强度分别在1.0 × 10−8-1.0 × 10− 6 moldm − 3和1.0 × 10−9-1.0 × 10− 7 moldm − 3范围内与药物浓度成线性关系。莱克多巴胺和非诺特罗的检出限分别为1.0 × 10− 10 mol dm− 3和1.0 × 10− 11 mol dm− 3。基于化学发光反应的动力学特征、化学发光光谱变化和自由基捕获实验，提出了Ni（IV）-鲁米诺-莱克多巴胺/非诺特罗化学发光体系中自由基参与的化学发光反应机理。讨论了莱克多巴胺/非诺特罗对Ni（IV）-鲁米诺化学发光体系的增敏机理。
AbstractA new, simple but efficient chemiluminescence system has been developed for determination of two β2-agonist drugs, ractopamine and fenoterol. The system is based on the chemiluminescence reaction of a Ni(IV) complex with luminol in alkaline media, while ractopamine or fenoterol can dramatically enhance chemiluminescence intensities. Under optimum conditions, chemiluminescence intensities are proportional to the concentrations of the drugs in the ranges of 1.0 × 10−8–1.0 × 10−6mol dm−3for ractopamine and of 1.0 × 10−9–1.0 × 10−7mol dm−3for fenoterol, respectively. The limits of detection (3σ) are 1.0 × 10−10mol dm−3for ractopamine and 1.0 × 10−11mol dm−3for fenoterol. Based on the kinetic characteristics of the chemiluminescence reaction, the chemiluminescence spectral change and free radical trapping experiment, a chemiluminescence reaction mechanism is proposed which involves free radicals in the Ni(IV)-luminol-ractopamine/fenoterol chemiluminescence systems. The mechanism of chemiluminescence enhancements by ractopamine/fenoterol to the Ni(IV)-luminol detection system is discussed.Graphical abstract