Synergistic Bacteria Killing through Photodynamic and Physical Actions of Graphene Oxide/Ag/Collagen Coating.
Synergistic Bacteria Killing through Photodynamic and Physical Actions of Graphene Oxide/Ag/Collagen Coating.
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DOI：
10.1021/acsami.7b06702
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发表时间：
2017-07
影响因子：
9.5
通讯作者：
Xianzhou Xie;Congyang Mao;Xiangmei Liu;Yanzhe Zhang;Z. Cui;Xian-Jin Yang;K. Yeung;H. Pan;P. C
中科院分区：
文献类型：
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作者：
Xianzhou Xie;Congyang Mao;Xiangmei Liu;Yanzhe Zhang;Z. Cui;Xian-Jin Yang;K. Yeung;H. Pan;P. C
研究人员普遍认为，银纳米粒子 (AgNP) 的广谱抗菌活性主要归因于 Ag+ 的作用。这项研究标志着第一份详细介绍AgNPs快速高效协同杀菌的报告，这是通过激发AgNPs强大的光催化能力，利用660 nm可见光快速产生自由基氧物种以及Ag+的固有抗菌能力来实现的。通过 Ag+ 的原位还原，这些 AgNP 均匀分布在轮廓分明的氧化石墨烯 (GO) 纳米片中，随后用 I 型胶原蛋白薄层包裹。体内皮下测试表明，660 nm可见光照射20分钟，种植体表面对大肠杆菌和金黄色葡萄球菌的抗菌功效分别达到96.3%和99.4%。此外，胶原蛋白可以降低涂层可能的细胞毒性。这项工作的结果可以提供一种高效、通用的基于GO的生物平台，与具有优异光催化性能的无机抗菌纳米粒子（即AgNPs）相结合，可用于简便快速的原位消毒，并通过660 nm光激发的光动力作用及其固有的协同杀菌作用来长期预防细菌感染。 物理抗菌能力。
Researchers have widely agreed that the broad spectrum antibacterial activity of silver nanoparticles (AgNPs) can be predominantly ascribed to the action of Ag+. This study marks the first report detailing the rapid and highly efficient synergistic bacteria killing of AgNPs, which is achieved by inspiring AgNPs' strong photocatalytic capability to rapidly produce radical oxygen species using 660 nm visible light together with the innate antimicrobial ability of Ag+. These AgNPs were uniformly distributed into well-defined graphene oxide (GO) nanosheets through an in situ reduction of Ag+ and subsequently wrapped with a thin layer of type I collagen. In vivo subcutaneous tests demonstrated that 20 min irradiation of 660 nm visible light could achieve a high antibacterial efficacy of 96.3% and 99.4% on the implant surface against Escherichia coli and Staphylococcus aureus, respectively. In addition, the collagen could reduce the coatings' possible cytotoxicity. The results of this work can provide a highly effective and universal GO-based bioplatform for combination with inorganic antimicrobial NPs (i.e., AgNPs) with excellent photocatalytic properties, which can be utilized for facile and rapid in situ disinfection, as well as long-term prevention of bacterial infection through the synergistic bacteria killing of both 660-nm light-inspired photodynamic action and their innate physical antimicrobial ability.