The report of my death was an exaggeration: A review for researchers using microsatellites in the 21st century.
The report of my death was an exaggeration: A review for researchers using microsatellites in the 21st century.
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DOI：
10.3732/apps.1600025
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发表时间：
2016-06
影响因子：
3.6
通讯作者：
Soltis PS
中科院分区：
文献类型：
--
作者：
Hodel RG;Segovia-Salcedo MC;Landis JB;Crowl AA;Sun M;Liu X;Gitzendanner MA;Douglas NA;Germain-Aubrey CC;Chen S;Soltis DE;Soltis PS
微卫星，或简单序列重复序列（SSR），长期以来一直在遗传研究中发挥着重要作用，由于其典型的高多态性。它们有不同的应用，包括基因组作图，法医学，确定亲子关系，种群和保护遗传学，鉴定多倍体的亲子关系，和地理学。我们比较了SSR和较新的方法，如测序基因分型（GBS）和限制性位点相关DNA测序（RAD-Seq），并为研究人员考虑使用哪些遗传标记提供建议。我们还回顾了目前用于识别微卫星位点和开发引物的各种技术，特别关注那些利用下一代测序（NGS）的技术。此外，我们审查软件的微卫星开发和报告的实验，以评估目前可用的软件SSR开发的效用。最后，我们讨论了微型卫星的未来，并为准备使用微型卫星的研究人员提出建议。我们认为，微卫星仍然有一个重要的位置，在基因组时代，因为它们仍然是有效的和具有成本效益的标记。
Microsatellites, or simple sequence repeats (SSRs), have long played a major role in genetic studies due to their typically high polymorphism. They have diverse applications, including genome mapping, forensics, ascertaining parentage, population and conservation genetics, identification of the parentage of polyploids, and phylogeography. We compare SSRs and newer methods, such as genotyping by sequencing (GBS) and restriction site associated DNA sequencing (RAD-Seq), and offer recommendations for researchers considering which genetic markers to use. We also review the variety of techniques currently used for identifying microsatellite loci and developing primers, with a particular focus on those that make use of next-generation sequencing (NGS). Additionally, we review software for microsatellite development and report on an experiment to assess the utility of currently available software for SSR development. Finally, we discuss the future of microsatellites and make recommendations for researchers preparing to use microsatellites. We argue that microsatellites still have an important place in the genomic age as they remain effective and cost-efficient markers.