Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum
Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum
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DOI：
10.1016/j.foodchem.2007.08.021
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发表时间：
2008-03-01
期刊：
影响因子：
8.8
通讯作者：
Wang, Yuan-Xing
中科院分区：
文献类型：
--
作者：
Chen, Yi;Xie, Ming-Yong;Wang, Yuan-Xing
从黑灵芝（Ganoderma atrum）子实体中提取了一种水溶性蛋白结合多糖。采用红外光谱、气相色谱、分子排阻色谱、氨基酸分析仪和高效液相色谱等手段对该蛋白的一级结构特征和分子量进行了表征。结果表明，该糖蛋白含有10.1%的蛋白质和17种氨基酸，富含谷氨酸、天冬氨酸、丙氨酸、甘氨酸、苏氨酸和丝氨酸。其主要由甘露糖、半乳糖和葡萄糖组成，摩尔比为1：1.28：4.91，平均分子量约为1013 kDa。通过β-消除反应证明PSG-1（多糖）中存在O-糖苷键。采用1，1-二苯基-2-苦基-肼（DPPH）自由基清除法和1，2，3-酚三醇自氧化法对纯化多糖进行体外抗氧化活性评价。这些不同的抗氧化活性与标准抗氧化剂维生素C和BHT进行了比较。结果表明，纯化后的多糖清除自由基的能力随浓度的增加而增强。结果表明，纯化多糖具有较强的清除DPPH自由基和超氧阴离子自由基的活性。本研究表明，纯化的多糖有可能作为天然抗氧化剂。(c)2007爱思唯尔有限公司保留所有权利。
A water-soluble protein-bound polysaccharide was extracted from the fruiting bodies of Ganoderma atrum and isolated by gel-filtration chromatography. Its primary structural features and molecular weight were characterized by infrared spectrometry, gas chromatography, size exclusion chromatography, amino acid analyzer and high-performance liquid chromatography (HPLC). The data obtained indicated that the glycoprotein contains 10.1% of protein and 17 general amino acids and it is rich in glutamic acid, asparagic acid, alanine, glycine, threonine, and serine. It was mainly composed of mannose, galactose and glucose in a molar ratio of 1:1.28:4.91, with an average molecular weight of about 1013 kDa. The existence of an O-glycosidic linkage in PSG-1 (polysaccharidel) was demonstrated by a beta-elimination reaction. The antioxidant activity of the purified polysaccharides was evaluated in vitro by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay, self-oxidation of 1,2,3-phentriol assay. Those various antioxidant activities were compared to standard antioxidants vitamin C and BHT. It was found that the scavenging effects of the purified polysaccharides increased with measuring concentration. The results indicated that the purified polysaccharides showed strong DPPH free radical and superoxide anion radical scavenging activities. This study suggested that the purified polysaccharides could potentially be used as natural antioxidants. (c) 2007 Elsevier Ltd. All rights reserved.