CD30 defines a subset of activated human T cells that produce IFN-gamma and IL-5 and exhibit enhanced B cell helper activity.
CD30 defines a subset of activated human T cells that produce IFN-gamma and IL-5 and exhibit enhanced B cell helper activity.
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DOI：
10.4049/jimmunol.153.7.2861
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发表时间：
1994-10
影响因子：
4.4
通讯作者：
M. Alzona;Hans-Martin Jäck;R. Fisher;T. M. Ellis
中科院分区：
文献类型：
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作者：
M. Alzona;Hans-Martin Jäck;R. Fisher;T. M. Ellis
CD30是一种淋巴细胞活化抗原，由活化的B淋巴细胞和T淋巴细胞以及选定的淋巴细胞恶性肿瘤表达。在T细胞中，CD30的表达仅限于少数（15 - 25%）活化的CD45RO+ T细胞。研究通过鉴定CD30+ T细胞产生细胞因子的特征，并通过评估它们帮助B细胞产生Ig的能力，来定义CD30+ T细胞的独特功能特性。通过逆转录- pcr （RT-PCR）评估细胞因子mRNA转录物，发现ifn - γ mRNA转录物仅存在于CD30+ T细胞中，这些细胞来自活化的CD45RO+ T细胞纯化的FACS。ifn - γ mRNA既不存在于活化的CD45RO+ T细胞的CD30-亚群中，也不存在于活化的CD45RA+ T细胞中。CD30+和CD30-亚群在激活3天后均表达IL-10和IL-2R α mrna，但此时均未表达IL-4或IL-6转录物。此外，活化的CD30+CD45RO+细胞（564 +/- 250 pg/ml）分泌的ifn - γ显著高于CD30-CD45RO+ (50 +/- 33 pg/ml)或CD45RA+ (0 +/- 0) T细胞。CD30+ T细胞也表达IL-5 mRNA，分泌的IL-5（320 +/- 73）水平显著高于活化的CD30- cd45ro + (17 +/- 5) T细胞。相比之下，CD30- T细胞产生的IL-2水平明显高于CD30+ T细胞（1270 +/- 380 vs 450 +/- 128）。在CD30+ T细胞中诱导ifn - γ并不是分离过程中使用的抗体与CD30结合的结果，尽管已知具有激动剂活性的抗CD30 Ab （M44）在固定到塑料中时能够诱导T细胞产生ifn - γ。最后，CD30+CD4+ T细胞比CD30-CD4+ T细胞表现出更大的辅助B细胞Ig生成活性。这些结果表明，CD30识别了T细胞的一个独特亚群，该亚群包括T细胞室中主要的ifn - γ和il -5产生细胞，并对B细胞产生Ig表现出强大的辅助活性。
CD30 is a lymphoid activation Ag expressed by activated B and T lymphocytes, as well as selected lymphoid malignancies. In T cells, CD30 expression is limited to a minority (15 to 25%) of activated CD45RO+ T cells. Studies were undertaken to define unique functional properties of CD30+ T cells by identifying the profile of cytokine production by CD30+ T cells, and by assessing their ability to provide help for B cell Ig production. Assessment of cytokine mRNA transcripts by reverse transcription-PCR (RT-PCR) revealed the presence of IFN-gamma mRNA transcripts only in CD30+ T cells derived from FACS purified from activated CD45RO+ T cells. IFN-gamma mRNA was present neither in the CD30- subset of activated CD45RO+ T cells, nor in activated CD45RA+ T cells. Both CD30+ and CD30- subsets expressed IL-10 and IL-2R alpha mRNAs after 3 days of activation, but neither population expressed IL-4 or IL-6 transcripts at this time. Furthermore, production of secreted IFN-gamma was significantly greater in activated CD30+CD45RO+ cells (564 +/- 250 pg/ml) than in CD30-CD45RO+ (50 +/- 33 pg/ml) or CD45RA+ (0 +/- 0) T cells. CD30+ T cells also expressed IL-5 mRNA and secreted significantly higher levels of IL-5 (320 +/- 73) than activated CD30-CD45RO+ (17 +/- 5) T cells. In contrast, CD30- T cells produced significantly higher levels of IL-2 than CD30+ T cells (1270 +/- 380 vs 450 +/- 128). Induction of IFN-gamma in CD30+ T cells was not a result of CD30 engagement by Abs used during the isolation process, although an anti-CD30 Ab (M44) known to exert agonist activity was capable of inducing IFN-gamma production by T cells when immobilized to plastic. Finally, CD30+CD4+ T cells exhibit significantly greater helper activity for B cell Ig production than CD30-CD4+ T cells. These results demonstrate that CD30 identifies a unique subset of T cells that comprise the major IFN-gamma- and IL-5-producing cells in the T cell compartment, and exhibit potent helper activity for B cell Ig production.