Immunostaining of UVA-induced DNA damage in erythrocytes of medaka ( Oryzias latipes )
Immunostaining of UVA-induced DNA damage in erythrocytes of medaka ( Oryzias latipes )
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UVA 诱导的青鳉 (Oryzias latipes) 红细胞 DNA 损伤的免疫染色
DOI：
10.1016/j.jphotobiol.2017.04.032
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Mitani Hiroshi
中科院分区：
文献类型：
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作者：
Sayed Alaa El-Din Hamid;Mitani Hiroshi
一些作者最近报道UVA会诱导DNA双链断裂（DSB）。只有少数研究人员报道了UVA暴露后DSB的诱导，如使用彗星试验和γ-H2 AX作为DSB形成的标志物所测量的。在本研究中，我们已经调查了第一次的剂量依赖性诱导的DSB的UVA青（Oryzias latipes）红细胞。成年雌性青鳉鱼暴露于UVA为15，30和60分钟/天，连续三天;未照射的对照组保持在相同的实验室条件。UVA照射后0 h和24 h采血检测DNA损伤和修复情况。UVA照射后0 h，γ-H2 AX灶数高于对照组，24 h内逐渐减少。彗星试验表明，DNA修复开始于恢复期。这些发现证实了我们先前的发现，遗传毒性效应后，UVA暴露在青鳉红细胞，并建议，复制独立的UVA诱导的DSB的形成是通过产生活性氧介导的。总之，这些结果表明，DNA损伤和修复发生后，UVA暴露在青鳉鱼。UVA是太阳紫外线辐射的主要成分，用于人工紫外线照射。我们的研究结果可能对皮肤癌研究有意义。
Some authors have recently reported that UVA induces double-strand breaks (DSBs) in DNA. Only a few researchers have reported on the induction of DSBs upon UVA exposure, as measured using the Comet assay and γ-H2AX as markers of DSB formation. In the present study, we have investigated for the first time the dose-dependent induction of DSBs by UVA in medaka (Oryzias latipes) erythrocytes. Adult female medaka fish were exposed to UVA for 15, 30, and 60 min/day for three continuous days; an unirradiated control group was kept in the same laboratory conditions. At 0 h and 24 h after UVA exposure, blood was collected to detect DNA damage and repair. The number of γ-H2AX foci was higher than the control value at 0 h after UVA exposure and decreased within a 24 h. the comet assay showed that DNA repair began during the recovery period. These findings confirm our pervious findings of genotoxic effects after UVA exposure in medaka erythrocytes and suggest that the replication-independent formation of UVA-induced DSBs is mediated through the generation of reactive oxygen species. In conclusion, these results suggest that DNA damage and repair occur after UVA exposure in medaka fish. UVA is the main component of solar UV radiation and is used for artificial UV exposure. Our results may have implications for skin cancer research.