ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE
ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE
复制标题
DOI：
10.1016/s0022-2836(65)80004-3
复制
发表时间：
1965-01-01
影响因子：
5.6
通讯作者：
THOMAS, CA
中科院分区：
文献类型：
--
作者：
BERNS, KI;THOMAS, CA
流感嗜血杆菌的核DNA补体已被确定为700至800 × 106道尔顿。已经开发了一种分离程序，该程序可获得最高分子量为400 X 106的DNA的高产率，或核DNA补体的一半。在转化分析中获得了先前未连锁标记的连锁。分离程序已扩展到大肠杆菌和粘质沙雷氏菌。
The nuclear DNA complement of Hemophilus influenzae has been determined to be 700 to 800 X 106 daltons. An isolation procedure has been developed which gives high yields of DNA with a maximum molecular weight of 400 X 106, or one-half of the nuclear DNA complement. Linkage of previously unlinked markers has been obtained in transformation assays. The isolation procedure has been extended to Escherichia coli and Serratia marcescens.