Mucosa-Associated Microbiota in Patients with Irritable Bowel Syndrome: A Comparison of Subtypes
Mucosa-Associated Microbiota in Patients with Irritable Bowel Syndrome: A Comparison of Subtypes
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DOI：
10.1159/000512167
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发表时间：
2020-12-03
期刊：
影响因子：
3.2
通讯作者：
Naito, Yuji
中科院分区：
文献类型：
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作者：
Matsumoto, Hiroshi;Shiotani, Akiko;Naito, Yuji
背景：大多数关于肠易激综合征（IBS）肠道微生物群的研究都集中在粪便微生物群，而不是粘膜相关微生物群（MAM）。目的：本研究的目的是调查IBS患者的MAM，包括IBS亚型的差异，即腹泻型IBS（IBS-D）和便秘型IBS（IBS-C）。方法：从 IBS 患者（17 名 IBS-D 患者和 7 名 IBS-C 患者）和 10 名健康对照者的回肠末端和乙状结肠采集内镜刷样本。通过 16S rRNA 基因扩增子测序来分析样品的 MAM。使用 PICRUSt 软件和 KEGG 数据库评估了 MAM 在功能层面的潜在变化。结果：根据基于 UniFrac 距离的 β 多样性，回肠末端和乙状结肠之间的 MAM 组成没有差异。鉴于 α 多样性，IBS-C 和 IBS-D 的乙状结肠 MAM 中的 Shannon（均匀度）而非 Chao1（丰富度）或观察到的操作分类单位往往低于对照组。乙状结肠中的4个属和回肠末端的7个属的丰度在3组之间存在显着差异。线性判别分析效应大小（LEfSe）显示，IBS-C组中瘤胃球菌属、阿克曼氏菌属、丁酸弧菌属、甲基杆菌属和微杆菌属以及丹毒菌科的丰度显着较高，链球菌属、氨基酸球菌属、丁酸球菌属和细单胞菌属的丰度显着较高。 IBS-D 组中较高。此外，IBS-D组负责分泌系统和LPS生物合成的基因比例显着高于IBS-C组，而负责甲烷代谢、赖氨酸生物合成和酶家族的基因比例显着低于IBS-C组。结论：IBS 亚型之间的生态失调模式和微生物组功能似乎有所不同，MAM 可能在 IBS 症状的产生中发挥着至关重要的作用。
Background: Most studies on gut microbiome of irritable bowel syndrome (IBS) have focused on fecal microbiota, instead of mucosa-associated microbiota (MAM). Aims: The aim of this study wasto investigate the MAM in IBS patients including the difference in subtypes of IBS, namely, diarrhea-predominant IBS (IBS-D) and constipation-predominant IBS (IBS-C). Methods: Endoscopic brush samples were taken from terminal ileum and sigmoid colon of patients with IBS (17 IBS-D patients and 7 IBS-C patients) and 10 healthy controls. The MAM of samples was profiled by 16S rRNA gene amplicon sequencing. Potential changes in the MAM at the functional level were evaluated using PICRUSt software and the KEGG database. Results: There were no differences in MAM composition between terminal ileum and sigmoid colon according to beta-diversity based on the UniFrac distance. In view of alpha-diversity, Shannon (evenness) but not Chao1 (richness) or observed operational taxonomic units tended to be lower in sigmoid colon MAM of IBS-C and IBS-D than the control group. The abundance of 4 genera in the sigmoid colon and 7 genera in the terminal ileum was significantly different among the 3 groups. Linear discriminant analysis effect size (LEfSe) showed that the genera of Ruminococcus, Akkermansia, Butyrivibrio, Methylobacterium, and Microbacterium and the family Erysipelotrichaceae were significantly higher in the IBS-C group, and the abundance of the genera Streptococcus, Acidaminococcus, Butyricicoccus, and Parvimonas was significantly higher in the IBS-D group. In addition, the proportion of genes responsible for the secretion system and LPS biosynthesis was significantly higher and that for methane metabolism, lysine biosynthesis, and enzyme families was significantly lower in the IBS-D group than in the IBS-C group. Conclusion: Dysbiosis pattern and the function of the microbiome seem to be different among subtypes of IBS, and MAM may play a crucial role in IBS symptom generation.