GD3 glycosphingolipid contributes to Fas-mediated apoptosis via association with ezrin cytoskeletal protein
GD3 glycosphingolipid contributes to Fas-mediated apoptosis via association with ezrin cytoskeletal protein
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DOI：
10.1016/s0014-5793(01)02776-4
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发表时间：
2001-09-28
期刊：
影响因子：
3.5
通讯作者：
Malorni, W
中科院分区：
文献类型：
--
作者：
Giammarioli, AM;Garofalo, T;Malorni, W
Fas 介导的淋巴样细胞凋亡的效率除其他方式外，还通过涉及其与埃兹蛋白（属于 4.1 蛋白家族的细胞骨架蛋白）相关的机制进行调节。在目前的工作中，我们为 Fas 触发细胞凋亡中与埃兹蛋白相关的另一种分子（二唾液酸神经节苷脂 GD3）提供了证据。事实上，作为一个早期事件，GD3 在尾足动物的膜相关结构域中重新分布，并与埃兹蛋白共定位。免疫共沉淀分析证实了这一结果，表明 GD3-ezrin 存在关联。总而言之，这些结果暗示了 GD3 在 Fas/ezrin 介导的细胞凋亡中的作用，支持尾足动物含有参与 Fas 介导的细胞凋亡的多分子信号复合物的观点。 (C) 2001 年欧洲生化学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
Efficiency of Fas-mediated apoptosis of lymphoid cells is regulated, among other means, by a mechanism involving its association with ezrin, a cytoskeletal protein belonging to the 4.1 family of proteins. In the present work, we provide evidence for a further molecule that associates to ezrin in Fas-triggered apoptosis, the disialoganglioside GD3. In fact, as an early event, GD3 redistributed in membrane-associated domains in uropods and co-localized with ezrin. Co-immunoprecipitation analyses confirmed this result, indicating a GD3-ezrin association. Altogether, these results are suggestive for a role of GD3 in Fas/ezrin-mediated apoptosis, supporting the view that uropods contain a multimolecular signaling complex involved in Fas-mediated apoptosis. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.