Juvenile hormone biosynthesis in diapause and nondiapause females of the adult blow fly Protophormia terraenovae
Juvenile hormone biosynthesis in diapause and nondiapause females of the adult blow fly Protophormia terraenovae
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DOI：
10.2108/zsj.20.1199
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发表时间：
2003-10-01
影响因子：
0.9
通讯作者：
Numata, H
中科院分区：
文献类型：
--
作者：
Shiga, S;Hamanaka, Y;Numata, H
用放化法测定了地果蝇滞育雌虫和生殖雌虫体内保幼激素（JH）的体外合成活性。薄层层析结果表明，鸡胚（CA）的产物与JH Ⅲ双环氧化合物的合成样品共迁移，但与JH Ⅲ和法呢酸甲酯均不共迁移。随着卵巢的发育，LD 18：6在25 ℃下饲养的雌性中JH合成活性增加。在LD 12：12和20 ℃条件下饲养的雌虫合成活性较低。在生殖条件下，切除大脑间部完全阻止了卵巢的发育，而在滞育诱导条件下，切除外侧部则导致卵巢的部分或全部发育。在这些手术动物中，JH合成活性与未手术和假手术动物无显著差异。结果表明，陆地棉CA主要产生JH Ⅲ型双环氧化合物，JH Ⅲ型双环氧化合物产生速率的降低是诱导滞育的原因。PI神经元和PL神经元在大脑中不直接介导的JH生产率的变化，但调节卵巢发育与一些未知的allatostatic和allatotropic因素合作。
In vitro synthetic activities of juvenile hormones (JH) were examined using a radiochemical assay in diapause females and reproductive females of the blow fly, Protophormia terraenovae. Thin layer chromatography showed that products of the corpus allatum (CA) comigrated with a synthetic sample of JH III bisepoxide but neither with JH III nor methylfarnesoate. JH synthetic activities increased in females reared under LD 18:6 at 25degreesC, as the ovaries developed. The synthetic activities remained low in previtellogenic females reared under LD 12:12 at 20degreesC. Removal of the pars intercerebralis compelely prevented ovaries from development under reproductive conditions, and removal of the pars lateralis caused partial or full development of ovaries under diapause-inducing conditions. In these operated animals, the JH synthetic activities were not significantly different from those of the intact and sham-operated animals. The results indicate that the CA in P terraenovae produces mainly JH III bisepoxide and a decrease in the JH production rate is a cause of diapause induction. PI eurons and PL neurons in the brain do not directly mediate changes in the JH production rate, but regulate ovarian development cooperatively with some unknown allatostatic and allatotropic factors.