The porcine reproductive and respiratory syndrome virus requires trafficking through CD163-positive early endosomes, but not late endosomes, for productive infection
The porcine reproductive and respiratory syndrome virus requires trafficking through CD163-positive early endosomes, but not late endosomes, for productive infection
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DOI：
10.1007/s00705-009-0527-1
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发表时间：
2009-12-01
影响因子：
2.7
通讯作者：
Nauwynck, Hans J.
中科院分区：
文献类型：
--
作者：
Van Gorp, Hanne;Van Breedam, Wander;Nauwynck, Hans J.
猪繁殖与呼吸综合征病毒（PRRSV）通过网格蛋白介导的内吞作用进入靶细胞。使用显性阴性Rab5和Rab7突变体，我们表明，在内化后，PRRSV进入早期内体，但不继续通过内吞途径到晚期内体。这通过共定位实验证实，共定位实验可视化了PRRSV和内吞途径的不同隔室的标志物。此外，显示PRRSV与其内化受体唾液酸粘附素共定位在细胞表面上和质膜下，而CD 163和PRRSV仅在早期内体中相遇。
The porcine reproductive and respiratory syndrome virus (PRRSV) enters its target cell via clathrin-mediated endocytosis. Using dominant-negative Rab5 and Rab7 mutants, we show that upon internalization, PRRSV enters early endosomes but does not continue through the endocytic pathway to late endosomes. This was confirmed via colocalization experiments visualizing PRRSV and markers for different compartments of the endocytic pathway. Furthermore, it was shown that PRRSV colocalizes with its internalization receptor, sialoadhesin, on the cell surface and beneath the plasma membrane, while CD163 and PRRSV only meet in early endosomes.