BLB8, an antiviral protein from Brevibacillus laterosporus strain B8, inhibits Tobacco mosaic virus infection by triggering immune response in tobacco
BLB8, an antiviral protein from Brevibacillus laterosporus strain B8, inhibits Tobacco mosaic virus infection by triggering immune response in tobacco
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DOI：
10.1002/ps.6472
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发表时间：
2021-05-26
影响因子：
4.1
通讯作者：
Chen, Huamin
中科院分区：
文献类型：
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作者：
Li, Yanfang;Jiao, Yubing;Chen, Huamin
背景烟草花叶病毒（Tobacco mosaic virus，TMV）是一种毁灭性的植物病毒，在世界范围内造成严重的经济损失。利用抗病毒蛋白或诱导子抑制病毒侵染或提高植物免疫力是防治TMV的有效策略之一。本实验室前期研究发现侧孢短芽孢杆菌B8菌株发酵液对烟草花叶病毒具有较强的抗病毒活性。结果BLB 8（B.侧孢菌菌株B8蛋白，BLB 8），一种来自B的抗病毒蛋白。侧孢霉菌株B8的分离和表征。BLB 8对TMV具有保护、抑制和治疗作用，抑制率分别达到63%、83%和55%。BLB 8在重组病毒TMV-GFP侵染部位周围浸润，抑制了TMV在体内的扩散和移动。用BLB 8预处理下部叶片可抑制TMV在上部叶片的传播和积累。此外，BLB 8还能剂量依赖性地诱导本氏烟草产生过敏反应，促进H2 O2的积累，并诱导烟草防御相关基因的表达。侧孢B8菌株通过引发烟草的植物免疫，有效地抑制了TMV的侵染，具有灭活、保护和治疗作用。因此，本研究为预防和控制病毒性疾病提供了一种新的抗病毒药物。
BACKGROUND Tobacco mosaic virus (TMV) is one of destructive plant viruses, causing serious economic losses in the world. Using antiviral proteins or elicitors to inhibit viral infection or promote plant immunity is one of the efficient strategies against TMV. Our previous study identified that the fermentation broth of Brevibacillus laterosporus strain B8 showed strong antiviral activity against TMV. However, the active antiviral ingredient is still unclear.RESULTS Here, BLB8 (B. laterosporus strain B8 protein, BLB8), an antiviral protein from B. laterosporus strain B8 was isolated and characterized. BLB8 showed protective, inactive and curative effects against TMV, and the inhibition rate reached up to 63%, 83% and 55%, respectively. BLB8 infiltrated around the infection site of the recombinant virus TMV-GFP inhibited the systemic extend and movement of TMV. Pretreatment of the bottom leaves with BLB8 inhibited the spread and accumulation of TMV in upper systemic leaves. Furthermore, BLB8 caused hypersensitive response (HR) in a dose-dependent way, promoted H2O2 accumulation, and induced the expression of defense-relative genes in Nicotiana benthamiana.CONCLUSION The antiviral protein BLB8 from B. laterosporus strain B8 effectively inhibits TMV infection in inactivation, protective and curative effects through triggering plant immunity in tobacco. Therefore, the present study provides a new antiviral agent for prevention and control of viral disease.