Genetic mapping of the nulliplex-branch gene (gb_nb1) in cotton using next-generation sequencing
Genetic mapping of the nulliplex-branch gene (gb_nb1) in cotton using next-generation sequencing
复制标题
使用下一代测序对棉花中的零分支基因 (gb_nb1) 进行遗传图谱分析
DOI：
10.1007/s00122-014-2452-2
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发表时间：
2015-03-01
影响因子：
5.4
通讯作者：
Zhang, Yongshan
中科院分区：
文献类型：
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作者：
Chen, Wei;Yao, Jinbo;Zhang, Yongshan
利用基于新一代测序的混合分组分析法，隐性无枝基因被定位在相距600kb的两个单核苷酸多态性（SNP）标记之间。在一种“无枝”棉花突变体中，大多数花朵直接从主茎的叶腋处长出，主茎通常没有果枝。无枝是研究棉花株型的一个有用性状；然而，该突变体的遗传基础一直不清楚。在本研究中，混合分组分析结合新一代测序技术被用于精细定位导致无枝植株的相关基因。无枝的比马棉品种新海18号与正常分枝的陆地棉品系TM - 1杂交，产生了F₂群体。发现无枝性状受隐性基因gb_nb1控制。通过亲本间的简化基因组测序发现了等位单核苷酸多态性（SNP），并且在利用F₂植株构建的无枝和正常枝混合群体中对其特征进行了描述。一个包含42个SNP标记、长9.0Mb的候选区域被发现与gb_nb1相关，通过F₂群体的分离分析，这有助于将其定位在16号染色体上600kb的区间内。本研究中开发的与gb_nb1紧密连锁的标记将有助于无枝性状的标记辅助选择，构建的精细图谱将加速gb_nb1的图位克隆。
Using bulked segregant analysis based on next-generation sequencing, the recessive nulliplex-branch gene was mapped between two SNP markers 600 kb apart.In a "nulliplex-branch" cotton mutant, most of the flowers arise directly from leaf axils on the main shoot, which usually does not have a fruiting branch. A nulliplex-branch is a useful trait by which to study cotton architecture; however, the genetic basis of this mutant has remained elusive. In this study, bulked segregant analysis combined with next-generation sequencing technology was used to finely map the underlying genes that result in a nulliplex-branch plant. The nulliplex-branch Pima cotton variety, Xinhai-18, was crossed with the normal branch upland cotton line, TM-1, resulting in an F-2 population. The nulliplex-branch trait was found to be controlled by the recessive gene gb_nb1. Allelic single-nucleotide polymorphisms (SNPs) were discovered by reduced-representation sequencing between the parents, and their profiles were also characterized in the nulliplex-branch and normal branch bulks constructed using the F-2 plants. A candidate 9.0 Mb-long region comprising 42 SNP markers was found to be associated with gb_nb1, which helped localize it at the 600-kb interval on Chr 16 by segregation analysis in the F-2 population. The closely linked markers with gb_nb1 developed in this study will facilitate the marker-assisted selection of the nulliplex-branch trait, and the fine map constructed will accelerate map-based cloning of gb_nb1.