Surface exposure and protease insensitivity of Borrelia burgdorferi Erp (OspEF-related) lipoproteins
Surface exposure and protease insensitivity of Borrelia burgdorferi Erp (OspEF-related) lipoproteins
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DOI：
10.1099/00221287-147-4-821
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发表时间：
2001-04-01
期刊：
影响因子：
2.8
通讯作者：
Stevenson, B
中科院分区：
文献类型：
--
作者：
El-Hage, N;Babb, K;Stevenson, B
伯氏疏螺旋体可以编码 Erp 家族的多种脂蛋白。尽管最初被描述为外表面蛋白质，但早期研究中使用的技术已被证明可以破坏细菌膜并允许标记次表面蛋白质。现在提供的额外分析数据表明 Erp 蛋白确实暴露在外膜表面。这些感染相关蛋白的表面定位表明 Erp 蛋白与脊椎动物组织相互作用的可能性。一些 Erp 蛋白对某些蛋白酶的原位消化具有抵抗力，表明这些蛋白以隐藏蛋白酶切割位点的方式折叠，或者它们与其他保护膜成分相互作用。此外，在存在针对 Erp 蛋白的抗体的情况下培养伯氏疏螺旋体可抑制细菌生长。
Borrelia burgdorferi can encode numerous lipoproteins of the Erp family. Although initially described as outer surface proteins, the technique used in that earlier study has since been demonstrated to disrupt bacterial membranes and allow labelling of subsurface proteins. Data are now presented from additional analyses indicating that Erp proteins are indeed surface exposed in the outer membrane. Surface localization of these infection-associated proteins indicates the potential for interactions of Erp proteins with vertebrate tissues, Some Erp proteins were resistant to in situ digestion by certain proteases, suggesting that those proteins fold in manners which hide protease cleavage sites, or that they interact with other protective membrane components. Additionally, cultivation of B. burgdorferi in the presence of antibodies directed against Erp proteins inhibited bacterial growth.