DAF-16 target genes that control C-elegans life-span and metabolism
DAF-16 target genes that control C-elegans life-span and metabolism
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DOI：
10.1126/science.1083614
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发表时间：
2003-04-25
期刊：
影响因子：
56.9
通讯作者：
Ruvkun, G
中科院分区：
文献类型：
--
作者：
Lee, SS;Kennedy, S;Ruvkun, G
从β 2/胰岛素受体到β 16/FOXO转录因子的信号传导控制不同门的寿命、代谢和发育。为了鉴定介导daf-2/胰岛素样信号传导的保守生物学输出的基因，我们使用比较基因组学鉴定了来自小杆线虫和果蝇的17个直链淀粉基因，每个基因在启动子区都有一个β-16结合位点。在C. elegans和候选基因的RNA干扰失活表明，这些基因中的许多介导daf-16功能的不同方面，包括寿命、代谢和发育。
Signaling from the DAF-2/insulin receptor to the DAF-16/FOXO transcription factor controls longevity, metabolism, and development in disparate phyla. To identify genes that mediate the conserved biological outputs of daf-2/insulin-like signaling, we used comparative genomics to identify 17 orthologous genes from Caenorhabditis and Drosophila, each of which bears a DAF-16 binding site in the promoter region. One-third of these DAF-16 downstream candidate genes were regulated by daf-2/insulin-like signaling in C. elegans, and RNA interference inactivation of the candidates showed that many of these genes mediate distinct aspects of daf-16 function, including longevity, metabolism, and development.