MODEL OF CALCIUM MOVEMENTS DURING ACTIVATION IN THE SARCOMERE OF FROG SKELETAL-MUSCLE
MODEL OF CALCIUM MOVEMENTS DURING ACTIVATION IN THE SARCOMERE OF FROG SKELETAL-MUSCLE
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DOI：
10.1016/s0006-3495(84)84238-1
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发表时间：
1984-01-01
影响因子：
3.4
通讯作者：
ALLEN, DG
中科院分区：
文献类型：
--
作者：
CANNELL, MB;ALLEN, DG
本文描述了一种蛙骨骼肌激活过程中钙离子运动的模型。该模型是基于半肌节的肌原纤维，并包括车厢代表终端池，纵向肌浆网，肌原纤维外空间和肌原纤维空间。钙结合蛋白肌钙蛋白，小清蛋白和钙螯合蛋白存在于适当的位置和现实的结合动力学。在激活过程中，一个时间依赖性的渗透性在终端池壁导致钙释放到肌浆和其扩散通过肌浆纵向和径向计算。在调整3个参数后，该模型产生的肌浆游离钙浓度与用钙指示剂实验记录的浓度非常相似。该模型已被用来证明小清蛋白在骨骼肌的松弛的重要性，描述的时间过程和幅度的钙梯度与跨肌节的扩散和估计的误差与使用水母发光蛋白作为细胞内钙指标在肌肉中。
A model of Ca movement during activation of frog skeletal muscle is described. The model was based on the half sarcomere of a myofibril and included compartments representing the terminal cisternae, the longitudinal sarcoplasmic reticulum, the extramyofibrillar space and the myofibrillar space. The Ca-binding proteins troponin, parvalbumin and calsequestrin were present in appropriate locations and with realistic binding kinetics. During activation, a time-dependent permeability in the terminal cisternal wall led to Ca release into the myoplasm and its diffusion through the myoplasm longitudinally and radially was computed. After adjustment of 3 parameters, the model produced a myoplasmic free-Ca concentration that was very similar to those recorded experimentally with Ca indicators. The model has been used to demonstrate the importance of parvalbumin in the relaxation of skeletal muscle, to describe the time course and magnitude of Ca gradients associated with diffusion across the sarcomere and to estimate the errors associated with the use of aequorin as an intracellular Ca indicator in muscle.