EVIDENCE FOR RELEASE OF SOLUBLE, BUT NOT OF MEMBRANE-INTEGRATED, PROTEINS FROM HUMAN-PLATELET ALPHA-GRANULES
EVIDENCE FOR RELEASE OF SOLUBLE, BUT NOT OF MEMBRANE-INTEGRATED, PROTEINS FROM HUMAN-PLATELET ALPHA-GRANULES
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DOI：
10.1016/0167-4838(82)90029-2
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
SOLUM, NO
中科院分区：
文献类型：
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作者：
GOGSTAD, GO;HAGEN, I;SOLUM, NO
从受刺激的血小板中释放的蛋白质与定义明确的α制剂的蛋白质进行了比较。-颗粒和可溶性细胞质的交叉免疫电泳。-颗粒中几乎检测到所有可释放蛋白；可溶性细胞质的真蛋白未被释放。释放的糖蛋白与凝集素的相互作用类似于它们的。α。-granula-located同行。.alpha。-用超声法将颗粒分离成可溶物和膜，然后进行超离心。可溶性含量的蛋白质与受刺激的血小板释放的蛋白质相对应。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳也支持这一观察结果。受刺激的血小板释放的大部分蛋白质来源于α -颗粒的可溶性成分。两个主要的。-颗粒抗原和肌球蛋白H链在. α中未被释放和恢复。颗粒膜。α释放的假设胞吐机制。支持来自血小板的-颗粒蛋白。
Proteins released from stimulated platelets were compared to those of a well-defined preparation of .alpha.-granules and the soluble cytoplasm by crossed immunoelectrophoresis. Nearly all releasable proteins were detected in the .alpha.-granule; the true proteins of the soluble cytoplasm were not released. The released glycoproteins interactd with lectins similarly to their .alpha.-granula-located counterparts. The .alpha.-granules were divided into soluble contents and membranes by ultrasonication followed by ultracentrifugation. The proteins of the soluble content corresponded to those released from the stimulated platelets. This observation was also supported by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The bulk of the proteins released from stimulated platelets originate from the soluble content of the .alpha.-granules. Two major .alpha.-granule antigens as well as the myosin H chain were not released and recovered in the .alpha.-granule membrane. The hypothetical exocytosis mechanism for the release of .alpha.-granule proteins from platelets was supported.